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Table 2 Nucleotide sequences of SSR markers for post-PCR labeling.

From: A post-labeling method for multiplexed and multicolored genotyping analysis of SSR, indel and SNP markers in single tube with bar-coded split tag (BStag)

Marker (DDBJ ID)

Motif

 

BStag/dye

Sequence (5' to 3')

SSR08A04

(aaac)n

Forward

F9GCC/VIC

CTAGTATTAGGACGCC CAAACACTTATTCGGGATCAG

(DY293653)

 

Reverse

 

gtttctt AATGCCATTATCAAACCGCC

SSR08A09

(cagg)n

Forward

F9GTC/PET

CTAGTATGAGGACGTC CATCATTGCATCAGCATCAC

(CX663959)

 

Reverse

 

gtttctt AGTTCTACATCATAACCTGCC

SSR08B15

(acc)n

Forward

G9GAC/NED

CTAGTATCAGGACGAC CCCATACTTGAAACCAAACC

(DY292389)

 

Reverse

 

gtttctt AGCAGCATGACTTAATCCA

SSR08B25

(tta)n

Forward

F9TAC/6-FAM

CTAGTATCAGGACTAC CGTTGACTCTCAAGAGATCTG

(DY289996)

 

Reverse

 

gtttctt CTTCACAGTCCGCAGCATTA

  1. Each marker was provided for genotyping with a BStag primer labeled with fluorescent dye. The nucleotide sequences of the attached BStag primer in the forward primers are underlined. The nucleotide sequences underlined and small capitals represent the 'pig-tail' sequence [14].