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Table 3 Nucleotide sequences of SNP markers attached with BStag sequences for post-PCR labeling.

From: A post-labeling method for multiplexed and multicolored genotyping analysis of SSR, indel and SNP markers in single tube with bar-coded split tag (BStag)

Marker (DDBJ ID)

SNP

Type

BStag/dye

Sequence (5' to 3')

LLL0729 (DC891931)

(C/G)

LSO

-

gtttctt GAAGTAGAAGGATGATAATCAC

  

ASO/C

F9GAC/NED

CTAGTATCAGGACGAC ATGGACACAACTCAAGTt C

  

ASO/G

F9GTC/PET

CTAGTATGAGGACGTC AATGGACACAACTCAAGTt G

MAPGR75R (DC893380)

(A/T)

LSO

-

gtttcttGGTTGGTATATCTACGAGAC

  

ASO/T

F9GTC/PET

CTAGTATGAGGACGTC TATCCGATTGAGCCTTTc T

  

ASO/A

F9TAC/6-FAM

CTAGTATCAGGACTAC ATCCGATTGAGCCTTTc A

  1. The underlined nucleotide sequences in lower case represent the 'pig-tail' sequence in LSO. The underlined nucleotide sequences in upper case represent the BStag attached to ASO. The nucleotide in bold type at the 3' end of each ASO corresponds to a polymorphic nucleotide, and the adjacent underlined lower case character represents the mismatched nucleotide. A set of LSO and ASO primers were used for PCR reaction with corresponding fluorescently labeled BStag primers.