Figure 1From: Glycine-rich RNA binding protein of Oryza sativa inhibits growth of M15 E. coli cellsDesign of constructs, confirmation of transcription and translation of rice proteins in E. coli. a. Linear maps of the recombinant constructs employed in this study. b. RT-PCR analysis of IPTG un-induced and induced samples of transformed E. coli M15 cells. Upper panel shows RT-PCR depicting expression of the respective genes. Lower panel shows EtBr stained gel depicting comparable staining of the ribosomal RNA species, 2 μg of total RNA was loaded in each lane. c. Expression of the foreign proteins in the transformed E. coli cells. Total protein from the IPTG un-induced and induced cells were separately analyzed. Induced proteins are indicated by arrows. Pre-stained markers are shown on the left side of the panel. UI and I refer to IPTG un-induced and induced samples, respectively.Back to article page