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Figure 3 | BMC Research Notes

Figure 3

From: Apolipoprotein A-I, A-II, and H mRNA and protein accumulation sites in the developing lung in late gestation

Figure 3

Distribution of apolipoprotein H mRNA and protein in the mouse fetal lung. Mouse tissue sections are from pseudoglandular (GD 15.5: f-h) or late canalicular (GD 17.5: a-e, i-l) stages. In situ hybridization (within blue frames) (a-e) was performed with apoH anti-sense (a, c-e) and sense (b) probes. Positive signals (blue) were found on GD 17.5 in the distal and proximal epithelia and smooth muscles surrounding large arteries. Immunohistochemistry (within red frames) (f-l) was performed using an anti-apoH polyclonal antibody (f-g, i-k) or goat IgG as negative control (h, l). Positive signals (red) were mainly found on capillaries with no change in localization between GD 15.5 and 17.5. GDs are indicated on photographs. Dashed frames, negative controls. Scale bars, 50 μm (a-b, e-f, h-l) or 20 μm (c-d, g). A, artery; C, capillaries; D, distal epithelium; M, mesenchyme; P, proximal epithelium; SM smooth muscle.

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