The spectrum of low molecular weight alpha-amylase/protease inhibitor genes expressed in the US bread wheat cultivar Butte 86

Table 2 Association of Butte 86 contigs with alpha-amylase/protease inhibitors identified by MS/MS in a previous proteomic analysis of Butte 86 flour

Butte 86 Contig	# Peptides¹	# Unique Peptides²	Spot Number³	MS/MS ID⁴
WMAI Bu-1	11	2	289, 528	[PRF:223520]⁵
WMAI Bu-2	9	0	nd	na
WDAI Bu-1/WDAI Bu-2⁶	9	2	280, 283, 285, 312*, 313	[SwissProt:P01085]⁵
WDAI Bu-3	9	3	281, 283*	TC11_338524
WDAI Bu-4	12	3	283, 286*	[Genbank:AAV91972]
WTAI-CM1 Bu-1	6	3	312, 313*	TC11_340510
WTAI-CM2 Bu-1	9	5	280, 285	[SwissProt:P16851]
WTAI-CM3 Bu-1	16	6	264, 265, 272	[SwissProt:P17314]
WTAI-CM3 Bu-2	14	4	264, 265	RS_UWI_15430
WTAI-CM16 Bu-1	17	12	266, 282, 284, 286	[SwissProt:P16159]
WTAI-CM17 Bu-1	11	6	274, 282	[Genbank:CAA42453]
WASI Bu-1⁷	12	1	244*	[SwissProt:P16347]⁵
WASI Bu-2	11	0	nd	na
CMx Bu-1	8	2	280, 285, 313	TC11_320696
CMx Bu-2	12	1	281, 286, 290*	TC11_308146
CMx Bu-3	14	2	281*, 290	TC11_309398
WTI Bu-1⁷	2	2	286⁸, 278	TC11_315743
WCI Bu-1	6	6	277, 278	[Genbank:CAD19440]

¹ Number of distinct peptides from Dupont et al. [17] that could be assigned to the protein encoded by Butte 86 contig.
² Number of peptides from Dupont et al. [17] that could be assigned exclusively to the protein encoded by Butte 86 contig.
³ Spot numbers from 2-DE analysis of Dupont et al. [17]. * indicates that the sequence was the predominant protein identified in the spot. nd indicates that unique peptides for the protein encoded by the contig were not detected in any spots.
⁴ MS/MS ID reported in Dupont et al. [17]. Accession numbers from DFCI Wheat Gene Index Release 11.0 are denoted with the prefix TC11_ and from HarvEST 1.14 are denoted with RS_UWI_. na indicates not applicable.
⁵ Protein sequence determined by Edman degradation.
⁶ Mature proteins encoded by WDAI Bu-1 and WDAI Bu-2 are identical.
⁷ Missing all or part of coding region for signal sequence.
⁸ Spot may contain an additional form of WTI not represented in Butte 86 EST collection.

ISSN: 1756-0500