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Table 1 Strains and plasmids used in this study.

From: Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice

Description or sequence  
Strain  
Corynebacterium pseudotuberculosis biovar ovis T1 (virulent strain isolates from goats; obtained from the Universidade Federal da Bahia, UFBA, Brazil).
MIC-6 (virulent strain isolate from goats; obtained from the Laboratório de Genética Celular e Molecular -LGCM, UFMG, Brazil).
E. coli TOP10 [F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-] Invitrogen.
B178 groEL44 mutant (ΔgroEL44) characterized by Zeilstra-Ryalls et al [19].
Plasmid  
pTopo® Cloning vector Cloning vector - ColE1/Apr - Invitrogen.
pVAX1© Vector Eukaryotic expression vector - pUC/Kmr - Invitrogen.
pTopo/hsp 60 Cloning vector with the C. pseudotuberculosis hsp60 gene inserted in the Bam HI and Hind III restriction sites of the vector.
pProEx-Hta/hsp 60 Prokaryotic expression vector containing the C. pseudotuberculosis hsp60 gene [13].
pVAX1/hsp 60 Eukaryotic expression vector containing the C. pseudotuberculosis hsp60 gene.