From: SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR
Dye | Concentration | R 2 | Slope | Efficiency |
---|---|---|---|---|
SYBR Green | 0.32 × (0.64 μM) | 0.985 | -3.40 | 97% |
SYBR Green | 1× (2 μM) | 0.395 | -2.13 | 195% |
EvaGreen | 1× | 0.995 | -3.30 | 101% |
EvaGreen | 5× | 0.999 | -3.46 | 95% |
SYTO 13 | 5 μM | 0.993 | -3.42 | 96% |
SYTO 13 | 10 μM | 0.993 | -3.44 | 95% |
SYTO 16 | 5 μM | 0.983 | -3.30 | 101% |
SYTO 16 | 10 μM | 0.992 | -3.43 | 96% |
SYTO 80 | 10 μM | 0.992 | -3.42 | 96% |
SYTO 80 | 20 μM | 0.986 | -3.41 | 96% |
SYTO 82 | 10 μM | 0.998 | -3.41 | 96% |
SYTO 82 | 20 μM | 0.996 | -3.37 | 98% |