A pilot study to evaluate the application of a generic protein standard panel for quality control of biomarker detection technologies

Table 1 The components and parameters of the proteins within the spike panels

Non-human Protein (with UniProt accession number)	Molecular Weight (kDa)	pI	Purity (%)	Spike concentration (pg/mL)
				Normal panel of generic standards	"Simulated diseased" panel of standards
Mouse_CCL6 P27784	10.7	9.38	> 97	50	100
Mouse_Lungkine Q9WVL7	16.5	7.07	> 90	10³	10³
Chicken_Caronte Q9PUK2	56	6.37	> 90	10⁴	10⁴
Mouse_Soggy Q9QZL9	26.8	7.18	> 90	10⁵	3 × 10⁵
Firefly_Luciferase Q27758	120	6.72	10-35	10⁶	6.67 × 10⁵
Chicken-Egg-White_Lysozyme P00698	14.7	9.36	≥ 90	10⁷	10⁷

The "normal" panel of generic standards was used for all work, whereas the "simulated diseased" panel of protein standards was incorporated only in the study to evaluate the ability to identify known changes in composition of the generic standards

ISSN: 1756-0500