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Table 2 Detection of compositional fold-changes in selected analytes between two panels of spike proteins

From: A pilot study to evaluate the application of a generic protein standard panel for quality control of biomarker detection technologies

Analyte

Ratio of signal outputs from the "normal" to "simulated diseased" panels of protein standards (n = 3)

Ratio of interpolated concentrations from the "normal" to "simulated diseased" panels of protein standards (n = 3)

 

Expected

Actual

Expected

Actual

CCL6

1:2

1: 1.26 ± 0.06

1:2

1: 1.72 ± 0.28

Lungkine

1:1

1: 0.94 ± 0.05

1:1

1: 1.71 ± 1.21

Caronte

1:1

1: 1.11 ± 0.21

1:1

1: 1.20 ± 0.29

Soggy

3:1

3: 1.02 ± 0.11

3:1

3: 0.96 ± 0.11

Luciferase

3:2

3: 1.80 ± 0.19

3:2

3: 1.86 ± 0.43

Lysozyme

1:1

1: 0.96 ± 0.06

1:1

1: 0.88 ± 0.32

  1. The ratios of each analyte between the "simulated normal and diseased" panels of proteins are tabulated below, in terms of signal outputs and interpolated concentrations. Theoretical ratios were calculated based on all analyte concentrations correlating with the linear working range of the assay. Theoretically within the "simulated diseased" panel, the concentration of CCL6 was 2-fold higher, whereas the concentrations of soggy and luciferase were 3-fold and 1.5-fold lower, respectively, compared with the same analytes within the "normal" generic panel of protein standards
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BMC Research Notes

ISSN: 1756-0500

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