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Figure 2 | BMC Research Notes

Figure 2

From: A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone

Figure 2

The α1p-rtTA mouse failed to transactivate the lamin A minigene. Western blot on bone protein extracts from bi-transgenic animals, tetop-LAG608G+;α1p-rtTA+ (lanes 2-4). Human lamin A/C (mab3211) and β-actin (A5441) primary antibodies were used. Protein extracts from a Hutchinson-Gilford progeria sample AG03506 (lane 1) and bone protein extracts from tetop-LAG608G+;Sp7-tTA+ mice (lane 6) and wild-type mice (lane 5) were included as positive and negative controls, respectively. Presence and absence of transgene are indicated by + and -, respectively

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