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Table 1 Primer pairs used to amplify the 3 target genes tilled in both EMS-mutant populations

From: Towards a TILLING platform for functional genomics in Piel de Sapo melons

Gene

Forward (*LabelledIRD700)

Reverse (*LabelledIRD800)

Cm-eIF4E

1st amplicon

GACTCAAACGCCTAACAGAAAATC

CTATCTCACCAAGTTTCCTAAATT

 

*GAGGGCGGTGCCATTCTTCTTCGG

*TCCCTAAATCGAACCAAGAAACGCC

Cm-eIF4E

2nd amplicon

TTGTTTCGTGTTGACATGTCCATC

AGCATGCATTTCACCTCATTGGCT

 

*TGCTTGGCTGTTAATTTATCTCTGC

*GTCAAGTACAGAACAAGAATCTGAG

Cm-eIF(iso)4E

GATCGATAATTTCCCTTTTC

TGACAGGCTTAAGCACTATG

 

*ACCCCAATTCATTCTAGGG

*TGTGCAAGCGCAACAAGGTAC

Cm-PDS

CAATGGGATGCTTAGATCAAC

TCCTTCCAAAATTACCCTAG

 

*GGATTTCTCGGAGTGACTCGG

*GCTCTCGAGCAACTAGCACT

  1. Nested PCR was used in all cases with labeled primers in the second PCR.