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Table 1 Evaluation of quality and quantity parameters of RNA samples extracted from SK-N-MC neuroblastoma.

From: Comparison of different methods for DNA-free RNA isolation from SK-N-MC neuroblastoma

Kit A230 A260 A280 A260/A280 A260/A230 Concentration (ng/μL) Elution volume (μL) Yield
(μg RNA/1E6 cells)
AxyPrep Multisource Total RNA Miniprep Kit (Axygen) 5.38 6.82 3.29 2.07 1.26 272.80 ± 28.55 100 3.94 ± 0.41
RNeasy® Mini Kit (Qiagen) 1.32 2.14 1.03 2.07 1.63 85.74 ± 72.26 50 0.62 ± 0.52
EasySpin (Citomed) 1.79 3.54 1.73 2.06 1.92 141.5 ± 74.58 50 1.02 ± 0.54
Illustra RNAspin Mini RNA Isolation Kit (GE) 0.3 0.15 0.07 2.13 0.37 5.98 ± 7.44 100 0.09 ± 0.11
TRIzol® Plus RNA Purification System (Invitrogen) 1.13 2.31 1.15 1.86 1.65 92.4 ± 111.50 50 0.67 ± 0.80
E.Z.N.A. ™ Total RNA Kit II (Omega bio-tek) 0.30 0.55 0.29 1.89 1.52 21.87 ± 4.14 50 0.16 ± 0.03