- Technical Note
- Open Access
Affinity-based enrichment strategies to assay methyl-CpG binding activity and DNA methylation in early Xenopus embryos
© Veenstra et al; licensee BioMed Central Ltd. 2011
Received: 17 June 2011
Accepted: 18 August 2011
Published: 18 August 2011
DNA methylation is a widespread epigenetic modification in vertebrate genomes. Genomic sites of DNA methylation can be bound by methyl-CpG-binding domain proteins (MBDs) and specific zinc finger proteins, which can recruit co-repressor complexes to silence transcription on targeted loci. The binding to methylated DNA may be regulated by post-translational MBD modifications.
A methylated DNA affinity precipitation method was implemented to assay binding of proteins to methylated DNA. Endogenous MeCP2 and MBD3 were precipitated from Xenopus oocyte extracts and conditions for methylation-specific binding were optimized. For a reverse experiment, DNA methylation in early Xenopus embryos was assessed by MBD affinity capture.
A methylated DNA affinity resin can be applied to probe for MBD activity in extracts. This assay has a broad application potential as it can be coupled to downstream procedures such as western blotting, fluorimetric HDAC assays and quantitative mass spectrometry. Methylated DNA affinity capture by methyl-CpG binding proteins produces fractions highly enriched for methylated DNA, suitable for coupling to next generation sequencing technologies. The two enrichment strategies allow probing of methyl-CpG protein interactions in early vertebrate oocytes and embryos.
DNA methylation is an epigenetic modification mostly associated with transcriptional repression in vertebrate genomes. In vertebrates, methylation events occur predominantly within the context of CpG dinucleotides, which in mammals by and large are methylated [1, 2]. The repressive signal inferred by DNA methylation is further interpreted by methyl-CpG binding domain (MBD) proteins that bind sites of DNA methylation and can recruit complexes with histone deacetylase (HDAC) activity to silence transcription [3–6]. The MBD was initially described as the minimal part of the MeCP2 protein required for methylated DNA binding . Four remaining family members (MBD1, MBD2, MBD3 and MBD4) have been discovered on the basis of homology searches with the MeCP2 MBD amino acid sequence . In addition, several structurally unrelated methyl-CpG binding proteins have been identified [9, 10]. Mutation or knockdown of DNA methyltransferases and MBD genes result in severe developmental phenotypes [11–14]. Mutations in the MBD family founder, MeCP2, are the cause of Rett Syndrome (RTT), a grave neurodevelopmental disorder which due to its X chromosome linkage almost exclusively affects females [15, 16]. In post-mitotic neurons, the brain-derived neurotrophic factor (BDNF) promoter III is controlled by phosphorylation-dependent MeCP2 binding [17, 18]. Membrane depolarization results in MeCP2 phosphorylation, carried out by the CamKI kinase, which diminishes its binding affinity for methylated DNA [19, 20]. A number of methods to study DNA-protein interactions have been developed over the years. For example, chromatin immunoprecipitation (ChIP) coupled to microarray hybridization or Next-Generation Sequencing technologies has facilitated the generation of genomic maps of transcription factor binding sites [21, 22]. Other widely used methods to study DNA-protein interactions involve DNA footprinting, EMSA (E lectrophoretic M obility S hift A ssay), southwestern blotting (SW), DNA affinity chromatography and DNA-protein crosslinking in vitro (DPC) [23, 24] DNA affinity precipitation is an efficient method for the analysis and purification of proteins displaying sequence specificity for DNA [25, 26]. Such assays can be used to study binding affinity and recruitment specificity of either recombinant or cellular proteins [27, 28]. Recently, important progress has been made in understanding the interactions of modified histone tails with transcriptional regulators [29–31]. These studies have combined peptide affinity pull-downs and quantitative mass spectrometry enabled by SILAC (S table I sotope L abeling by A mino Acids in C ell Culture) . It will be of great importance to define proteins recruited to methylated DNA during early development. For over a century amphibians from the genus Xenopus have been the organisms of choice to study developmental processes. The main reason for this is external embryonic development as well as the relatively large size of the embryos themselves which allows easy experimental manipulation. A number of studies involving DNA methylation and methyl-CpG binding proteins established Xenopus laevis as an important model for epigenetics studies [9, 33–35]. Sequencing of the Xenopus tropicalis genome rendered this organism suitable for (epi)genomics studies of the early vertebrate development [36–38]. Here we describe an efficient method for methylated DNA affinity precipitation of MBDs from Xenopus oocytes. Using in vitro methylated DNA oligonucleotides bound to magnetic beads and conditions that promote methylation-specific binding, MeCP2 and MBD3 were pulled down. These methylated DNA affinity precipitation assays have a broad application potential as they can be combined with a number of biochemical techniques and used in different model systems. Furthermore, to test the ability of MeCP2 to affinity-precipitate methylated DNA from early Xenopus embryos, the immobilized MBD domain of MeCP2 can be used to enrich for methylated genomic DNA [39–41]. The fractions enriched for methylated DNA, due to their high recovery rate, are suitable for coupling to next generation sequencing technologies.
Oocyte extract preparation
Xenopus laevis oocytes were collected in an eppendorf tube and homogenized in ice cold lysis buffer (20 mM Tris (pH = 8,0), 70 mM KCl, 1 mM EDTA, 10% glycerol, 5 mM DTT, 0,125% NP40, Roche Complete protease inhibitors). The extract was centrifuged for 5 minutes at 4°C at maximum speed. The supernatant was collected and quick-frozen in liquid nitrogen. The final oocyte concentration in the extract was 0.5 oocyte equivalents per μL assuming that one oocyte corresponds to 1 μL.
MeCP2-MBD in vitro transcription and translation
The pT7TS-5'MBD construct, encoding the first 176 amino acids of Xenopus laevis MeCP2, was obtained by PCR amplification (primers 5'-gtatccgtcgacaattcggcacgagagaaaATG and 5'-acaagaggatcctcaGGCTTTCGGTTGCTTCTGTTCC), digestion of the PCR product with SalI and BamH1, blunting and ligation in the EcoRV site of pT7TS. In vitro transcription of the pT7TS-5'MBD was performed using the mMESSAGE mMACHINE T7 kit (Ambion). The in vitro translation was achieved using 1 μg of RNA in a total volume of 50 μL (Flexi Rabbit, Promega). The binding assay was performed in 150 μL ABCD buffer 20 mM Tris (pH = 8,0), 1 mM EDTA, 5 mM DTT, 5 mM MgCl2, 100 mM KCl, 0,125% NP40, protease inhibitors) with 6 μL of the in vitro translation reaction, using the protocol described below.
DNA affinity precipitation
The DNA probe was obtained by annealing of 50 μg of two complementary oligonucleotides (5'-GATCCCGGAGTTAA and 5'-GATCTTAACTCCGG), modified from Klose et al. . The annealed probe was phosphorylated with T4 polynucleotide kinase (New England Biolabs) for 2 h at 37°C, purified by phenol-chlorophorm and precipitated with ethanol. The concatamerization reaction was performed at RT for 4 h with T4 ligase (Promega) in the presence of 10 mM ATP to replenish the ATP from the ligase buffer. The ligation process was monitored using 1% agarose gel electrophoresis. The reaction was ethanol precipitated for one hour at -20°C. After DNA precipitation, two biotinylated nucleotides, biotin-16-dUTP and biotin-14-dATP were used to fill in the overhangs in a polymerization reaction using the Klenow fragment (New England Biolabs). All the nucleotides in the reaction were present at a concentration of 0.1 mM. The reaction was allowed to proceed for 45 minutes at room temperature. The biotinylated DNA was then purified using a gel extraction kit (Qiagen) and the samples were recovered in 30 μl elution buffer. The biotinylated oligos (~50 μg) were methylated with SssI methyltransferase in a 500 μl reaction for 2 h at 37°C. Phenol-chlorophorm extraction was used to clean the methylated DNA which was then precipitated by ethanol. The methylated probe was then coupled to streptavidin beads (DYNA beads M-280). For each 3 μg of DNA, 30 μl of the streptavidin beads was used. The beads were washed 3X with the WB (1 M NaCl, 20 mM Tris pH 8.0, 1 mM EDTA) buffer. After washing, 3 μg of the methylated probe (10 μl) was mixed with 40 μl of WB buffer. Out of the 50 μl of the WB buffer, 10 μl were separated for subsequent gel analysis while the remaining 40 μL was added to streptavidin beads. The mixture was incubated on a thermomixer set at 30°C for 30 minutes with shaking. Oocyte extracts (100 μL) were coupled to the immobilized probe in affinity precipitation (AP) buffer (20 mM Tris pH 8.0, 1 mM EDTA, 5 mM DTT, 5 mM MgCl2, 100 mM KCl, 0.125% NP40, protease inhibitors) in a 1:1 ratio and left rotating over night at 4°C. After incubation the mixtures were washed with washing buffer (20 mM Tris (pH = 8,0), 1 mM EDTA, 5 mM DTT, 5 mM MgCl2, 100-200 mM KCl, 0,125% NP40, protease inhibitors) for three times and the bound fraction was eluted with 50 μL 1X Laemmli sample buffer and analyzed by western blotting.
The eluate (25 μl) was resolved on 10% polyacrylamide gels and subjected to western blotting. The α-MeCP2 antibody (V17-1) was raised against the C-terminal peptide MeCP2 peptide of Xenopus laevis (PRPTREEPVDTRTT). The final serum was affinity purified and used in a 1:1000 dilution. The ISWI, Mi-2 and MBD3 antibodies have been described before [5, 43, 44]. The western blotting protocol was performed as described before .
Genomic DNA isolation and sonication
Xenopus tropicalis embryos (n = 50) were homogenized in 625 μL (12.5 μl/embryo) of homogenization buffer (20 mM Tris pH 8.0, 100 mM NaCl, 15 mM EDTA, 1% SDS, 0.5 mg/ml proteinase K). The homogenate was then incubated for 3 h at 55°C. The tubes were gently inverted after every hour of incubation. Two Phenol/Chlorophorm/Isoamylalcohol (25:24:1, PCI) extractions were performed using the same amount (625 μL) of PCI and inverting the tubes gently. The samples were spun for 5 minutes at 13000 rpm in a table-top centrifuge following every PCI extraction. The DNA was precipitated by adding 1/5 volume of 4 M NH4Ac and 3 volumes of cold 96% ethanol and left on ice for 1 h. The DNA precipitate was then centrifuged for 20 minutes at 4°C and 13,000 rpm in a table-top centrifuge. The pellet was washed with 500 μL of 70% ethanol and centrifuged for 5 minutes, 13,000 rpm at RT. The pellet was then resuspended in 200 μL of TE buffer with 1 μl of RNAse A (20 μg/μl) and left at RT for 30 minutes. The reaction was precipitated with 0.1 volumes of 4 M NH4Ac and 1 volume of isopropanol on ice for 1 h. The precipitate was centrifuged for 20 minutes at 4°C at maximum speed in a table-top centrifuge and washed with 70% ethanol. The pellet was resuspended in 100 μl of DNAse/RNAse free water. Two samples consisting of 50 embryo equivalents were pulled together to obtain a 200 μl sample with a concentration of ~ 0.1 μg/μL. The sample was sonicated for 15 minutes (13 seconds on, 16 seconds off, maximum output) in a Bioruptor (Diagenode) device.
Methylated DNA affinity capture (MethylCap)
Genomic DNA was sheared by sonication to ~ 500 bp fragments. Methylated DNA was affinity purified using the H6-GST-MBD fusion protein (Diagenode) following the manufacturers' protocol with one modification. The DNA was eluted in several steps with increasing NaCl concentrations (150 μl): 2 × 200 mM, 1 × 300 mM, 1 × 500 mM, 1 × 600 mM and 1 × 700 mM. The recovery of methylated DNA from six genomic targets was calculated by quantitative PCR, whereas the concentration of DNA in each eluate was obtained by Picogreen measurement.
Immobilized DNA preparation
Binding of the in vitro translated MBD domain to immobilized templates
DNA affinity precipitation of MBDs from oocyte extracts
To optimize washing and elution conditions, the protein-DNA complexes formed during an overnight incubation of oocyte extracts and immobilized probes, were washed with increasing concentrations (100/200/400 mM) of KCl and eluted with Laemmli buffer. Once eluted with Laemmli buffer, the samples were spun down to separate the beads from the supernatant and the eluate was loaded on SDS-PAGE gel. Western blot analysis revealed strong preference for methylated DNA binding of both MBD3 and MeCP2 (Figure 2C-D). The mammalian MBD3 is not able to bind methylated DNA due to an insertion in the MBD. Xenopus MBD3, however, is present in a short and a long form, the former of which is able to specifically bind methylated CpGs [5, 47]. Whereas MeCP2 tolerates 200 mM KCl in the washing buffer, most of MBD3 is eluted at such conditions (Figure 2C). MeCP2 and the short form of MBD3 display a strong preference for methylated DNA (Figure 2D), in contrast to Mi-2 which can bind both methylated and unmethylated DNA. MBD3 and Mi-2 form part of the same complex ; the difference in binding may be explained by a variable composition of the Mi-2/NuRD complex [48–50] and methylation-independent binding of DNA by the Mi-2/NuRD subunit MTA1 . Finally, a negative control for DNA methylation pull-downs was established. Any endogenous protein that does not bind directly to DNA might serve as a negative control in these assays. TFIIB is one of the general transcription factors involved in RNA Polymerase II pre-initiation complex formation . Our assays identified it as a suitable negative control as it does not bind immobilized oligo templates independent of their methylation status (Figure 2E). In the same experiment we also tested the binding of ISWI, another evolutionarily conserved ATP-dependent chromatin binding protein and established its uniform binding to methylated and unmethylated DNA [44, 52] Altogether, these results establish Xenopus oocytes as an efficient system to probe for interactions of methylated DNA and proteins with methyl-CpG binding activity. Furthermore, we provide a number of controls that can be used in such assays to control for unspecific protein-DNA interactions.
Methylated DNA affinity capture (MethylCap)
In this study we describe an efficient method for the pull-down of DNA methylation-specific proteins from Xenopus oocyte extracts. Furthermore, we demonstrate the functionality of this system by specifically pulling down two major Xenopus methyl-CpG binding proteins. In addition, we assay the ability of the methyl-CpG binding domain of MeCP2 to affinity-capture methylated genomic DNA and establish conditions that result in the elution of methylated fractions. Using methylated DNA affinity precipitation, MeCP2 and MBD3 were pulled down from Xenopus oocytes and positive as well as negative controls for this approach were established. The chromatin remodelers Mi-2 and ISWI can be used as loading controls and TFIIB as a negative control. Methylated DNA affinity precipitation is a valuable technique that can be coupled to western blotting or HDAC assays in order to assess the composition of bound fractions. Also, a great potential lies in combining methylated DNA pull-downs with mass spectrometry approaches to aid the identification of proteins with DNA methylation-specific binding. A number of recent studies demonstrated the importance of similar applications in identifying novel chromatin components in human cells . The binding activity of methyl-CpG binding proteins in vertebrate oocytes/embryos has not yet been established. For example, in brain extracts, MeCP2 is known to be actively regulated by serine phosphorylation which diminishes its DNA binding affinity and enables the proper neural response [17, 19]. It is possible that during early embryogenesis similar phosphorylation events, which result in altered binding affinity, take place. Also, MBD proteins are known to interact with a plethora of proteins and most of these interactions are dependent on the biological context . Methylated DNA affinity precipitation coupled to mass spectrometry might help in identifying the nature of these interactions in the developing embryo. To capture methylated DNA, genomic DNA isolated from early Xenopus embryos was incubated with an MBD affinity resin and eluted with increasing salt concentrations. The fractions eluted at concentrations of 500 - 700 mM NaCl contain fragments of highly methylated DNA [38, 40, 41]. On the other hand, the majority of non-methylated fragments that were non-specifically bound to the resin already elute with 200 mM NaCl. The relatively high concentration of DNA in the fractions renders this method highly suitable for coupling to next generation sequencing technologies.
Binding of MBDs to sites of genomic DNA methylation is a biologically important process that modulates the transcriptional output of the cell. It is therefore essential to have suitable assays to probe for this kind of interactions. Together, the two affinity-capture based assays discussed above, allow for unbiased profiling of methylated DNA-binding proteins and the genomic loci they may target, setting the stage for a new and exciting era of quantitative profiling in early vertebrate embryos.
Statement on animal use
Animal care and use was in accordance with national and European guidelines and standard operating procedures approved by the institutional animal care and use committee (Dierexperimentencommissie, DEC).
This work was funded by the grant of the Netherlands Organization of Scientific Research (NWO-ALW VIDI grant 864.03.002) to GJCV.
- Bird AP: CpG-rich islands and the function of DNA methylation. Nature. 1986, 321 (6067): 209-213. 10.1038/321209a0.PubMedView ArticleGoogle Scholar
- Hendrich B, Tweedie S: The methyl-CpG binding domain and the evolving role of DNA methylation in animals. Trends Genet. 2003, 19 (5): 269-277. 10.1016/S0168-9525(03)00080-5.PubMedView ArticleGoogle Scholar
- Jones PL, Veenstra GJ, Wade PA, Vermaak D, Kass SU, Landsberger N, Strouboulis J, Wolffe AP: Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription. Nat Genet. 1998, 19 (2): 187-191. 10.1038/561.PubMedView ArticleGoogle Scholar
- Zhang Y, Ng HH, Erdjument-Bromage H, Tempst P, Bird A, Reinberg D: Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation. Genes Dev. 1999, 13 (15): 1924-1935. 10.1101/gad.13.15.1924.PubMedPubMed CentralView ArticleGoogle Scholar
- Wade PA, Gegonne A, Jones PL, Ballestar E, Aubry F, Wolffe AP: Mi-2 complex couples DNA methylation to chromatin remodelling and histone deacetylation. Nat Genet. 1999, 23 (1): 62-66. 10.1038/12664.PubMedView ArticleGoogle Scholar
- Nan X, Ng HH, Johnson CA, Laherty CD, Turner BM, Eisenman RN, Bird A: Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. Nature. 1998, 393 (6683): 386-389. 10.1038/30764.PubMedView ArticleGoogle Scholar
- Nan X, Meehan RR, Bird A: Dissection of the methyl-CpG binding domain from the chromosomal protein MeCP2. Nucleic Acids Res. 1993, 21 (21): 4886-4892. 10.1093/nar/21.21.4886.PubMedPubMed CentralView ArticleGoogle Scholar
- Hendrich B, Bird A: Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell Biol. 1998, 18 (11): 6538-6547.PubMedPubMed CentralView ArticleGoogle Scholar
- Ruzov A, Dunican DS, Prokhortchouk A, Pennings S, Stancheva I, Prokhortchouk E, Meehan RR: Kaiso is a genome-wide repressor of transcription that is essential for amphibian development. Development. 2004, 131 (24): 6185-6194. 10.1242/dev.01549.PubMedView ArticleGoogle Scholar
- Filion GJ, Zhenilo S, Salozhin S, Yamada D, Prokhortchouk E, Defossez PA: A family of human zinc finger proteins that bind methylated DNA and repress transcription. Mol Cell Biol. 2006, 26 (1): 169-181. 10.1128/MCB.26.1.169-181.2006.PubMedPubMed CentralView ArticleGoogle Scholar
- Li E, Bestor TH, Jaenisch R: Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell. 1992, 69 (6): 915-926. 10.1016/0092-8674(92)90611-F.PubMedView ArticleGoogle Scholar
- Okano M, Bell DW, Haber DA, Li E: DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell. 1999, 99 (3): 247-257. 10.1016/S0092-8674(00)81656-6.PubMedView ArticleGoogle Scholar
- Stancheva I, Meehan RR: Transient depletion of xDnmt1 leads to premature gene activation in Xenopus embryos. Genes Dev. 2000, 14 (3): 313-327.PubMedPubMed CentralGoogle Scholar
- Iwano H, Nakamura M, Tajima S: Xenopus MBD3 plays a crucial role in an early stage of development. Dev Biol. 2004, 268 (2): 416-428. 10.1016/j.ydbio.2003.12.032.PubMedView ArticleGoogle Scholar
- Amir RE, Van den Veyver IB, Wan M, Tran CQ, Francke U, Zoghbi HY: Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat Genet. 1999, 23 (2): 185-188. 10.1038/13810.PubMedView ArticleGoogle Scholar
- Shahbazian MD, Zoghbi HY: Molecular genetics of Rett syndrome and clinical spectrum of MECP2 mutations. Curr Opin Neurol. 2001, 14 (2): 171-176. 10.1097/00019052-200104000-00006.PubMedView ArticleGoogle Scholar
- Martinowich K, Hattori D, Wu H, Fouse S, He F, Hu Y, Fan G, Sun YE: DNA methylation-related chromatin remodeling in activity-dependent BDNF gene regulation. Science. 2003, 302 (5646): 890-893. 10.1126/science.1090842.PubMedView ArticleGoogle Scholar
- Chen WG, Chang Q, Lin Y, Meissner A, West AE, Griffith EC, Jaenisch R, Greenberg ME: Derepression of BDNF transcription involves calcium-dependent phosphorylation of MeCP2. Science. 2003, 302 (5646): 885-889. 10.1126/science.1086446.PubMedView ArticleGoogle Scholar
- Tao J, Hu K, Chang Q, Wu H, Sherman NE, Martinowich K, Klose RJ, Schanen C, Jaenisch R, Wang W, et al: Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function. Proc Natl Acad Sci USA. 2009, 106 (12): 4882-4887. 10.1073/pnas.0811648106.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhou Z, Hong EJ, Cohen S, Zhao WN, Ho HY, Schmidt L, Chen WG, Lin Y, Savner E, Griffith EC, et al: Brain-specific phosphorylation of MeCP2 regulates activity-dependent Bdnf transcription, dendritic growth, and spine maturation. Neuron. 2006, 52 (2): 255-269. 10.1016/j.neuron.2006.09.037.PubMedPubMed CentralView ArticleGoogle Scholar
- Smith AD, Sumazin P, Das D, Zhang MQ: Mining ChIP-chip data for transcription factor and cofactor binding sites. Bioinformatics. 2005, 21 (Suppl 1): i403-412. 10.1093/bioinformatics/bti1043.PubMedView ArticleGoogle Scholar
- Valouev A, Johnson DS, Sundquist A, Medina C, Anton E, Batzoglou S, Myers RM, Sidow A: Genome-wide analysis of transcription factor binding sites based on ChIP-Seq data. Nat Methods. 2008, 5 (9): 829-834. 10.1038/nmeth.1246.PubMedPubMed CentralView ArticleGoogle Scholar
- Guille MJ, Kneale GG: Methods for the analysis of DNA-protein interactions. Mol Biotechnol. 1997, 8 (1): 35-52. 10.1007/BF02762338.PubMedView ArticleGoogle Scholar
- Jiang D, Jarrett HW, Haskins WE: Methods for proteomic analysis of transcription factors. J Chromatogr A. 2009, 1216 (41): 6881-6889. 10.1016/j.chroma.2009.08.044.PubMedPubMed CentralView ArticleGoogle Scholar
- Kadonaga JT, Tjian R: Affinity purification of sequence-specific DNA binding proteins. Proc Natl Acad Sci USA. 1986, 83 (16): 5889-5893. 10.1073/pnas.83.16.5889.PubMedPubMed CentralView ArticleGoogle Scholar
- Gadgil H, Oak SA, Jarrett HW: Affinity purification of DNA-binding proteins. J Biochem Biophys Methods. 2001, 49 (1-3): 607-624. 10.1016/S0165-022X(01)00223-8.PubMedView ArticleGoogle Scholar
- Lombard-Platet G, Jalinot P: Purification by DNA affinity precipitation of the cellular factors HEB1-p67 and HEB1-p94 which bind specifically to the human T-cell leukemia virus type-I 21 bp enhancer. Nucleic Acids Res. 1993, 21 (17): 3935-3942. 10.1093/nar/21.17.3935.PubMedPubMed CentralView ArticleGoogle Scholar
- Vermeulen M, Stunnenberg HG: An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes. Biol Proced Online. 2004, 6: 157-162. 10.1251/bpo85.PubMedPubMed CentralView ArticleGoogle Scholar
- Vermeulen M, Mulder KW, Denissov S, Pijnappel WW, van Schaik FM, Varier RA, Baltissen MP, Stunnenberg HG, Mann M, Timmers HT: Selective anchoring of TFIID to nucleosomes by trimethylation of histone H3 lysine 4. Cell. 2007, 131 (1): 58-69. 10.1016/j.cell.2007.08.016.PubMedView ArticleGoogle Scholar
- Vermeulen M, Eberl HC, Matarese F, Marks H, Denissov S, Butter F, Lee KK, Olsen JV, Hyman AA, Stunnenberg HG, et al: Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers. Cell. 2010, 142 (6): 967-980. 10.1016/j.cell.2010.08.020.PubMedView ArticleGoogle Scholar
- Bartke T, Vermeulen M, Xhemalce B, Robson SC, Mann M, Kouzarides T: Nucleosome-interacting proteins regulated by DNA and histone methylation. Cell. 2010, 143 (3): 470-484. 10.1016/j.cell.2010.10.012.PubMedPubMed CentralView ArticleGoogle Scholar
- Ong SE, Foster LJ, Mann M: Mass spectrometric-based approaches in quantitative proteomics. Methods. 2003, 29 (2): 124-130. 10.1016/S1046-2023(02)00303-1.PubMedView ArticleGoogle Scholar
- Stancheva I, Collins AL, Van den Veyver IB, Zoghbi H, Meehan RR: A mutant form of MeCP2 protein associated with human Rett syndrome cannot be displaced from methylated DNA by notch in Xenopus embryos. Mol Cell. 2003, 12 (2): 425-435. 10.1016/S1097-2765(03)00276-4.PubMedView ArticleGoogle Scholar
- Dunican DS, Ruzov A, Hackett JA, Meehan RR: xDnmt1 regulates transcriptional silencing in pre-MBT Xenopus embryos independently of its catalytic function. Development. 2008, 135 (7): 1295-1302. 10.1242/dev.016402.PubMedView ArticleGoogle Scholar
- Ruzov A, Shorning B, Mortusewicz O, Dunican DS, Leonhardt H, Meehan RR: MBD4 and MLH1 are required for apoptotic induction in xDNMT1-depleted embryos. Development. 2009, 136 (13): 2277-2286. 10.1242/dev.032227.PubMedPubMed CentralView ArticleGoogle Scholar
- Akkers RC, van Heeringen SJ, Jacobi UG, Janssen-Megens EM, Francoijs KJ, Stunnenberg HG, Veenstra GJ: A hierarchy of H3K4me3 and H3K27me3 acquisition in spatial gene regulation in Xenopus embryos. Dev Cell. 2009, 17 (3): 425-434. 10.1016/j.devcel.2009.08.005.PubMedPubMed CentralView ArticleGoogle Scholar
- van Heeringen SJ, Akhtar W, Jacobi UG, Akkers RC, Suzuki Y, Veenstra GJ: Nucleotide composition-linked divergence of vertebrate core promoter architecture. Genome Res. 2011, 21 (3): 410-421. 10.1101/gr.111724.110.PubMedPubMed CentralView ArticleGoogle Scholar
- Bogdanovic O, Long SW, van Heeringen SJ, Brinkman AB, Gomez-Skarmeta JL, Stunnenberg HG, Jones PL, Veenstra GJ: Temporal uncoupling of the DNA methylome and transcriptional repression during embryogenesis. Genome Res. 2011Google Scholar
- Kangaspeska S, Stride B, Metivier R, Polycarpou-Schwarz M, Ibberson D, Carmouche RP, Benes V, Gannon F, Reid G: Transient cyclical methylation of promoter DNA. Nature. 2008, 452 (7183): 112-115. 10.1038/nature06640.PubMedView ArticleGoogle Scholar
- Bock C, Tomazou EM, Brinkman AB, Muller F, Simmer F, Gu H, Jager N, Gnirke A, Stunnenberg HG, Meissner A: Quantitative comparison of genome-wide DNA methylation mapping technologies. Nat Biotechnol. 2010, 28 (10): 1106-1114. 10.1038/nbt.1681.PubMedPubMed CentralView ArticleGoogle Scholar
- Brinkman AB, Simmer F, Ma K, Kaan A, Zhu J, Stunnenberg HG: Whole-genome DNA methylation profiling using MethylCap-seq. Methods. 2010, 52 (3): 232-236. 10.1016/j.ymeth.2010.06.012.PubMedView ArticleGoogle Scholar
- Klose RJ, Sarraf SA, Schmiedeberg L, McDermott SM, Stancheva I, Bird AP: DNA binding selectivity of MeCP2 due to a requirement for A/T sequences adjacent to methyl-CpG. Mol Cell. 2005, 19 (5): 667-678. 10.1016/j.molcel.2005.07.021.PubMedView ArticleGoogle Scholar
- Guschin D, Wade PA, Kikyo N, Wolffe AP: ATP-Dependent histone octamer mobilization and histone deacetylation mediated by the Mi-2 chromatin remodeling complex. Biochemistry. 2000, 39 (18): 5238-5245. 10.1021/bi000421t.PubMedView ArticleGoogle Scholar
- Kikyo N, Wade PA, Guschin D, Ge H, Wolffe AP: Active remodeling of somatic nuclei in egg cytoplasm by the nucleosomal ATPase ISWI. Science. 2000, 289 (5488): 2360-2362. 10.1126/science.289.5488.2360.PubMedView ArticleGoogle Scholar
- Veenstra GJ, Destree OH, Wolffe AP: Translation of maternal TATA-binding protein mRNA potentiates basal but not activated transcription in Xenopus embryos at the midblastula transition. Mol Cell Biol. 1999, 19 (12): 7972-7982.PubMedPubMed CentralView ArticleGoogle Scholar
- Jallow Z, Jacobi UG, Weeks DL, Dawid IB, Veenstra GJ: Specialized and redundant roles of TBP and a vertebrate-specific TBP paralog in embryonic gene regulation in Xenopus. Proc Natl Acad Sci USA. 2004, 101 (37): 13525-13530. 10.1073/pnas.0405536101.PubMedPubMed CentralView ArticleGoogle Scholar
- Saito M, Ishikawa F: The mCpG-binding domain of human MBD3 does not bind to mCpG but interacts with NuRD/Mi2 components HDAC1 and MTA2. J Biol Chem. 2002, 277 (38): 35434-35439. 10.1074/jbc.M203455200.PubMedView ArticleGoogle Scholar
- Le Guezennec X, Vermeulen M, Brinkman AB, Hoeijmakers WA, Cohen A, Lasonder E, Stunnenberg HG: MBD2/NuRD and MBD3/NuRD, two distinct complexes with different biochemical and functional properties. Mol Cell Biol. 2006, 26 (3): 843-851. 10.1128/MCB.26.3.843-851.2006.PubMedPubMed CentralView ArticleGoogle Scholar
- Passannante M, Marti CO, Pfefferli C, Moroni PS, Kaeser-Pebernard S, Puoti A, Hunziker P, Wicky C, Muller F: Different Mi-2 complexes for various developmental functions in Caenorhabditis elegans. PLoS One. 2010, 5 (10): e13681-10.1371/journal.pone.0013681.PubMedPubMed CentralView ArticleGoogle Scholar
- Yao YL, Yang WM: The metastasis-associated proteins 1 and 2 form distinct protein complexes with histone deacetylase activity. J Biol Chem. 2003, 278 (43): 42560-42568. 10.1074/jbc.M302955200.PubMedView ArticleGoogle Scholar
- Roeder RG: The role of general initiation factors in transcription by RNA polymerase II. Trends Biochem Sci. 1996, 21 (9): 327-335.PubMedView ArticleGoogle Scholar
- Guschin D, Geiman TM, Kikyo N, Tremethick DJ, Wolffe AP, Wade PA: Multiple ISWI ATPase complexes from xenopus laevis. Functional conservation of an ACF/CHRAC homolog. J Biol Chem. 2000, 275 (45): 35248-35255. 10.1074/jbc.M006041200.PubMedView ArticleGoogle Scholar
- Weber M, Davies JJ, Wittig D, Oakeley EJ, Haase M, Lam WL, Schubeler D: Chromosome-wide and promoter-specific analyses identify sites of differential DNA methylation in normal and transformed human cells. Nat Genet. 2005, 37 (8): 853-862. 10.1038/ng1598.PubMedView ArticleGoogle Scholar
- Mohn F, Weber M, Schubeler D, Roloff TC: Methylated DNA immunoprecipitation (MeDIP). Methods Mol Biol. 2009, 507: 55-64. 10.1007/978-1-59745-522-0_5.PubMedView ArticleGoogle Scholar
- Jia J, Pekowska A, Jaeger S, Benoukraf T, Ferrier P, Spicuglia S: Assessing the efficiency and significance of Methylated DNA Immunoprecipitation (MeDIP) assays in using in vitro methylated genomic DNA. BMC Res Notes. 2010, 3: 240-10.1186/1756-0500-3-240.PubMedPubMed CentralView ArticleGoogle Scholar
- Meissner A, Mikkelsen TS, Gu H, Wernig M, Hanna J, Sivachenko A, Zhang X, Bernstein BE, Nusbaum C, Jaffe DB, et al: Genome-scale DNA methylation maps of pluripotent and differentiated cells. Nature. 2008, 454 (7205): 766-770.PubMedPubMed CentralGoogle Scholar
- Lister R, Pelizzola M, Dowen RH, Hawkins RD, Hon G, Tonti-Filippini J, Nery JR, Lee L, Ye Z, Ngo QM, et al: Human DNA methylomes at base resolution show widespread epigenomic differences. Nature. 2009, 462 (7271): 315-322. 10.1038/nature08514.PubMedPubMed CentralView ArticleGoogle Scholar
- Martens JH, Brinkman AB, Simmer F, Francoijs KJ, Nebbioso A, Ferrara F, Altucci L, Stunnenberg HG: PML-RARalpha/RXR Alters the Epigenetic Landscape in Acute Promyelocytic Leukemia. Cancer Cell. 2010, 17 (2): 173-185. 10.1016/j.ccr.2009.12.042.PubMedView ArticleGoogle Scholar
- Cross SH, Charlton JA, Nan X, Bird AP: Purification of CpG islands using a methylated DNA binding column. Nat Genet. 1994, 6 (3): 236-244. 10.1038/ng0394-236.PubMedView ArticleGoogle Scholar
- Serre D, Lee BH, Ting AH: MBD-isolated Genome Sequencing provides a high-throughput and comprehensive survey of DNA methylation in the human genome. Nucleic Acids Res. 2010, 38 (2): 391-399. 10.1093/nar/gkp992.PubMedPubMed CentralView ArticleGoogle Scholar
- Bogdanovic O, Veenstra GJ: DNA methylation and methyl-CpG binding proteins: developmental requirements and function. Chromosoma. 2009, 118 (5): 549-565. 10.1007/s00412-009-0221-9.PubMedPubMed CentralView ArticleGoogle Scholar
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