Selection of reference genes for quantitative real-time PCR expression studies of microdissected reproductive tissues in apomictic and sexual Boechera

Table 1 Boechera-specific qRT-PCT primers for tested HKGs

Gene identification/Gene description	Primer sequence 5'-3' forward/reverse	Amplicon size (bp)	Amplification efficiency ± SD *	EMBL Accession Number
BoechACT2/Actin 2	GTTCCACCACTGAGCACAATGTTACC/AGTCTTGTTCCAGCCCTCTTTTGTG	132	0.94 ± 0.003	FR846456
BoechEF1/Elongation factor-1 alpha	CCAAGGGTGAAAGCAAGGAGAGC/CACTGGTGGTTTTGAGGCTGGTATCT	75	0.96 ± 0.002	FR846458
BoechRPS18/Ribosomal protein S18	GCTGGGGAGTTATCTGCTGCTGAG/CTTGCCGTCTTTGTAATCCTTCTGC	117	0.94 ± 0.003	FR846460
BoechPEX4/Peroxin 4	TTTGCAGTTGACAGTTGGATCTTGTTC/TCGCTCGTGATGCCTATTCATCATAC	143	0.83 ± 0.009	FR846459
BoechAt1g09770.1/Arabidopsis thaliana cell division cycle 5	GCCATGATCTAAAAAGTTGGGACAAA/TATTCGTCACAACACATGCAAGGTTTA	145	0.88 ± 0.007	FR846457
BoechUBQ/Polyubiquitine	GGCTAAGATCCAGGACAAGGAAGGTAT/CTGGATGTTATAGTCAGCCAAAGTGCG	71	0.94 ± 0.004	FR851958

*Amplification efficiency was calculated using the miner algorithm [30] for both the means and corresponding SD: $\bar{x} = σ^{2} \cdot \sum_{i = 1}^{n} \frac{1}{σ_{i}^{2}} x_{i}$ [39]

ISSN: 1756-0500