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Table 3 Summary of the 2 best HKG combinations for different tissues and reproductive system according to geNorm

From: Selection of reference genes for quantitative real-time PCR expression studies of microdissected reproductive tissues in apomictic and sexual Boechera

Tissue

Recommended HKGs

M values

V2/3 values

Recommended HKGs (Boech RPS18 replaced by UBQ)

M values

V2/3 values

Apo all tissues

Boech RPS18 Boech Efα1

0.130

0.042

Boech UBQ Boech Efα1

0.120

0.120

Apo vegetative tissues

Boech Efα1 Boech ACT2

0.170

0.007

Boech UBQ Boech Efα1

0.220

0.120

Apo ovules

Boech RPS18 Boech Efα1

0.060

0.040

Boech UBQ Boech Efα1

0.055

0.131

Apo anthers

Boech ACT2 Boech Efα1

0.020

0.001

Boech ACT2 Boech Efα1

0.020

0.090

Sex all tissues

Boech RPS18 Boech ACT2

0.017

0.078

Boech UBQ Boech ACT2

0.200

0.100

Sex vegetative tissues

Boech RPS18 Boech Efα1

0.070

0.089

Boech UBQ Boech ACT2

0.220

0.100

Sex ovules

Boech RPS18 Boech ACT2

0.010

0.088

Boech UBQ Boech Efα1

0.010

0.057

Sex anthers

Boech Efα1 Boech ACT2

0.001

0.025

Boech Efα1 Boech ACT2

0.001

0.016