Table 1 Yield and purity of DNA and RNA preparations from 0,8 mm cortex punches using commonly employed protocols, commercially available kits and the presented simultaneous DNA/RNA extraction method
From: Simultaneous DNA and RNA isolation from brain punches for epigenetics
Method DNA | Â | Total yield [ng] | 260/280 | 260/230 |
|---|---|---|---|---|
(n = 6) | Qiagen Dneasy | 360 ± 96 | 1,8 | 1,35 |
|  | CTAB | 520 ± 87 | 1,8 | 1,5 |
|  | SDS/Prot K | 850 ± 195 | 2 | 1,4 |
|  | Gentra Puregene | 360 ± 96 | 2 | 1,5 |
Method RNA | Â | Â | Â | Â |
(n = 6) | MN Nucleospin II | 366 ± 75 | 2,1 | 0,2 |
|  | Trizol | 690 ± 262 | 1,7 | 0,6 |
|  | Chomczynski | 838 ± 321 | 1,9 | 1,4 |
Simultaneous DNA/RNA | Â | Â | Â | Â |
(n = 9) | DNA | 765 ± 163 | 1,9 | 1,3 |
|  | RNA | 330 ± 54 | 1,9 | 1,2 |