Skip to main content
Figure 4 | BMC Research Notes

Figure 4

From: Microarray profiling reveals the integrated stress response is activated by halofuginone in mammary epithelial cells

Figure 4

Global expression profiling implicates multiple biological pathways downstream of HF. (A) Relative abundance of HF transcriptional targets based on biological ontogeny. Biological processes which accounted for one percent or more and p < 0.001 are specifically shown. Note that stress response and unfolded protein response characteristic genes are among those showing enrichment. Enrichment analysis was performed using The Database for Annotation, Visualization and Integrated Discovery (DAVID) v6.7. (B) Validation plot showing high correlation between array results and validation by quantitative RT-PCR. Fold-change values of 21 genes from the microarray (y-axis) are plotted against the corresponding values from the qRT-PCR (x-axis). Each value is the log2-ratio of the treated vs. the untreated samples. The slope of the regression line is 0.704 and the Pearson correlation coefficient is 0.938 (p-value 1.33e-11), indicating highly significant agreement.

Back to article page