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Figure 5 | BMC Research Notes

Figure 5

From: Microarray profiling reveals the integrated stress response is activated by halofuginone in mammary epithelial cells

Figure 5

HF upregulates the expression of Integrated Stress Response effector genes. (A) A heat-map of signature genes related to the Integrated Stress Response (ISR) is displayed. Among reported genes in previous studies, nineteen genes were chosen if absolute fold-change was greater than 2 and q-value was less than 0.001. Measurement values were normalized to have mean zero and variance one per gene. The heat-map was generated using matrix2png. (B) Validation of HF induction of selected ISR genes by quantitative RT-PCR (qRT-PCR). qRT-PCR was preformed on an independent set of samples from an 8 hour 0.01 μM HF or MAZ1310 treatment. qRT-PCR data is expressed as the mean fold change in the level of the indicated transcript between HF and MAZ1310 treated samples, and the standard error of the means (SEM) is included. Each condition was assayed in triplicate, and the experiment was repeated three times. Aggregate results are shown. (C) GSEA plot. Enrichment Score (ES) was 0.962 and its nominal P-value < 10-3. top: Enrichment profile where running ES for the Atf4/ISR gene set as the analysis walks along the ranked list; middle: The Hits shows where the members of the Atf4/ISR gene set appear in the ranked list; bottom: Ranked list metric measures a gene's correlation with a phenotype (i.e. Halofuginone treatment).

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