Figure 10
From: Selection of reference genes for qPCR in hairy root cultures of peanut
Optimal number of reference genes for normalization. Calculations were performed using pairwise variation, calculated on geNorm (A) and accumulated SD, calculated on NormFinder (B). For A: V2/3, pairwise variation between the two most stable genes (TBP2 and RPL8C) + 3rd most stable gene (HEL); V3/4, addition of the 4th most stable gene (TIP41); V4/5, addition of the 5th most stable gene (CYP1); V5/6, addition of the 6th most stable gene (EFα1); V6/7, addition of the 7th most stable gene (AT1G31300); V7/8, addition of the 8th most stable gene (ACT7); V8/9, addition of the 9th most stable gene (EIF4A1); V9/10, addition of the 10th most stable gene (GAPDH); V10/11, addition of the 11th most stable gene (H3); V11/12, addition of the 12th most stable gene (rolC). For B: 1, EFα1; 2, 1 + ACT7; 3, 2 + TBP2; 4, 3 + AT1G31300; 5, 4 + PRL8C; 6, 5 + CYP1; 7, 6 + TIP41; 8, 7 + HEL; 9, 8 + EIF4A1 ; 10, 9 + GAPDH; 11, 10 + rolC; 12, 11 + H3.