Table 1 Comparison of the 454 assemblies of barcoded BACs with their Sanger reference sequences.
From: Sequencing of BAC pools by different next generation sequencing platforms and strategies
BAC | Data sets1 | sequence depth | average read length (bp) | L50 (bp) | L80 (bp) | misass2 | gaps | total gap size (bp) | penalties3 |
|---|---|---|---|---|---|---|---|---|---|
bcFLX | 27 | 224 | 52,352 | 52,352 | 0 | 1 | 50 | 1 | |
184G09 120,562 bp | bcTi | 56 | 256 | 121,630 | 121,630 | 0 | 0 | 0 | 0 |
bcTids | 27 | 256 | 120,569 | 120,569 | 0 | 0 | 0 | 0 | |
bcFLX | 15 | 225 | 11,912 | 3,586 | 6 | 9 | 490 | 21 | |
259I16 124,050 bp | bcTi | 25 | 253 | 68,888 | 14,394 | 2 | 3 | 177 | 7 |
bcTids | 15 | 253 | 24,258 | 10,367 | 6 | 5 | 355 | 17 | |
bcFLX | 26 | 223 | 52,601 | 11,098 | 6 | 5 | 199 | 17 | |
631P08 101,158 bp | bcTi | 66 | 252 | 25,788 | 17,610 | 2 | 2 | 77 | 6 |
bcTids | 26 | 252 | 52,257 | 17,582 | 1 | 1 | 14 | 3 | |
bcFLX | 26 | 219 | 16,866 | 3,203 | 17 | 4 | 392 | 38 | |
711N16 112,178 bp | bcTi | 41 | 292 | 21,923 | 3,860 | 5 | 3 | 30 | 13 |
bcTids | 26 | 292 | 21,921 | 3,859 | 5 | 3 | 25 | 13 | |
bcFLX | 23 | 223 | 33,433 | 17,560 | 29 | 19 | 1,131 | 77 | |
all | bcTi | 47 | 263 | 59,557 | 39,374 | 9 | 8 | 284 | 26 |
bcTids | 23 | 263 | 54,751 | 38,094 | 12 | 9 | 394 | 33 |