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Table 2 Error rates of different chemistries by comparison to the Sanger reference sequences.

From: Sequencing of BAC pools by different next generation sequencing platforms and strategies

BAC

Data sets1

sequence depth

error rate

Q2

 

bcFLX

27

1.16E-04

39

184G09

bcTi

56

1.49E-04

38

 

bcTids

27

1.49E-04

38

 

bcFLX

15

4.08E-04

34

259I16

bcTi

25

3.49E-04

35

 

bcTids

15

4.40E-04

34

 

bcFLX

26

1.94E-04

37

631P08

bcTi

66

1.43E-04

38

 

bcTids

26

3.09E-04

35

 

bcFLX

26

1.66E-04

38

711N16

bcTi

41

5.24E-04

33

 

bcTids

26

5.00E-04

33

 

bcFLX

 

2.27E-04

36

all

bcTi

 

2.90E-04

35

 

bcTids

 

3.37E-04

35

  1. 1 bcFLX: barcoded FLX; bcTi: barcoded Titanium; bcTids: barcoded Titanium, down sampled to the same sequence depth as of bcFLX;
  2. 2 Q = -10*log(error rate).
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BMC Research Notes

ISSN: 1756-0500

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