Skip to main content

Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Table 3 Stability ranking of candidate reference genes in T cells, neutrophils and unselected blood leukocytes by NormFinder, geNorm and BestKeeper.

From: Selection of reliable reference genes for quantitative real-time PCR in human T cells and neutrophils

  T cells
Rank NormFinder geNorm BestKeeper
1 RPL13A RPL13A/IPO8 RPL13A
2 IPO8   TBP
3 TBP 18S IPO8
4 18S TBP SDHA
5 SDHA SDHA 18S
6 GAPDH GAPDH GAPDH
7 HBB HBB HBB
  Neutrophils
Rank NormFinder geNorm BestKeeper
1 ACTB SDHA/RPL13A TBP
2 TBP   18S
3 SDHA TBP SDHA
4 GAPDH ACTB ACTB
5 18S GAPDH GAPDH
6 RPL13A 18S RPL13A
  Total Blood Leukocytes
Rank NormFinder geNorm BestKeeper
1 SDHA SDHA/TBP 18S
2 TBP   TBP
3 IPO8 18S SDHA
4 GAPDH RPL13A RPL13A
5 RPL13A IPO8 IPO8
6 18S GAPDH GAPDH/ACTB
7 ACTB ACTB  
8 HBB HBB HBB
  1. Ranking is based on the stability values (NormFinder), the average expression stability during stepwise exclusion of the least stable gene (geNorm), or SD ± Cq (BestKeeper). Rank 1 represents the most stable reference gene or combination of reference genes. GeNorm analysis does not allow the ranking of the two most stable genes because its gene-stability measurements require the use of gene ratios.