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Table 1 Primers used for identification of glucose-6-phosphate dehydrogenase defectsa

From: Molecular characterization of glucose-6-phosphate dehydrogenase deficiency in Jeddah, Kingdom of Saudi Arabia

Analysis

Analyzed Region

Forward 5'→3'

Reverse 5'→3'

RFLP Analysis (Tm = 61°C)

Exon 3

GTGGAGGATGATGTATGTAGGT

AGGGCAGGGCACAGCTGTAA

 

Exon 4

TACAGTCGTGCCCTGCCCT

CCGAAGCTGGCCATGCTGG

 

Exon 5

CTGTGTGTGTGTCTGTCTGTC

GGAGGGCAACGGCAAGCCTT

 

Exon 6

GCAGCTGTGATCCTCACTCC

GCAAGGTGGAGGAACTGACC

Gene sequencing (Tm = 56°C)

Exons

1, 2

AAGCTCGGTAATGATAAGCACGC

TGGAGCAGGCACTTCCTGG

 

Exons

3, 4, 5

GTGGAGGATGATGTATGTAGGT

GGAGGGCAACGGCAAGCCTT

 

Exons

6, 7, 8

AGAGGGTCATCTGGGAACACAA

CCTGGGACATGACAACTTGGG

 

Exons

9, 10, 11, 12, 13

TCTGTGGCCACAGTCATCCC

CGCCCTCCTCCTTCCTTCTG

  1. Abbreviations: A, alanine; C, cysteine; G, glycine; RFLP, restriction fragment length polymorphism; Tm, Anealing temperature; T, threonine
  2. aAdapted from Moradkhani et al.[14]