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Table 3 Consensus ranking

From: Reference gene validation for qPCR in rat carotid body during postnatal development

Gene ranking for group N1+N14 (n = 16)

Rank Position

geNorm

NormFinder

BestKeeper

Consensus

1

RPII

GAPDH

PPIA

PPIA

2

TBP

PPIA

Actin

GAPDH

3

PPIA

Actin

GAPDH

Actin

4

Actin

TBP

18s

RPII

5

GAPDH

RPII

RPII

TBP

6

18s

18s

TBP

18s

Gene ranking for group N14+Hyper14 (n = 14)

Rank Position

geNorm

NormFinder

BestKeeper

Consensus

1

PPIA

RPII

RPII

RPII

2

TBP

GAPDH

PPIA

TBP

3

RPII

TBP

GAPDH

GAPDH

4

GAPDH

Actin

TBP

PPIA

5

Actin

PPIA

Actin

Actin

6

18s

18s

18s

18s

Gene ranking for group N14+Hypo14 (n = 14)

Rank Position

geNorm

NormFinder

BestKeeper

Consensus

1

PPIA

TBP

RPII

TBP

2

TBP

PPIA

PPIA

PPIA

3

RPII

GAPDH

TBP

RPII

4

GAPDH

RPII

Actin

GAPDH

5

Actin

18s

GAPDH

Actin

6

18s

Actin

18s

18s

Gene ranking for group N1+N14+Hyper14+Hypo14 (n = 28)

Rank Position

geNorm

NormFinder

BestKeeper

Consensus

1

PPIA

TBP

PPIA

TBP

2

TBP

GAPDH

TBP

PPIA

3

RPII

18s

GAPDH

GAPDH

4

GAPDH

PPIA

RPII

RPII

5

Actin

RPII

Actin

18s

6

18s

Actin

18s

Actin

  1. Weighted rank aggregation was performed to combine the ordered lists of genes produced by geNorm, NormFinder, and BestKeeper to a consensus rank of genes.