From: Reference gene validation for qPCR in rat carotid body during postnatal development
Gene and consensus ranking for group N1+N14 (n = 16) using Bio-Rad SYBR | |||||||
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Bio-Rad SYBR | |||||||
Rank Position | geNorm | M-value | NormFinder | Variability | BestKeeper | Coeff. of corr. [r] | Consensus |
1 | TBP | 0.26 | GAPDH | 0.01 | PPIA | 0.89 | PPIA |
2 | RPII | 0.26 | PPIA | 0.011 | Actin | 0.84 | GAPDH |
3 | PPIA | 0.27 | Actin | 0.014 | GAPDH | 0.83 | Actin |
4 | Actin | 0.34 | TBP | 0.015 | 18s | 0.73 | TBP |
5 | GAPDH | 0.38 | RPII | 0.016 | RPII | 0.72 | RPII |
6 | 18s | 0.40 | 18s | 0.018 | TBP | 0.70 | 18s |
Gene and consensus ranking for group N1 + N14 (n = 16) using Applied Biosystems SYBR | |||||||
Applied Biosystems SYBR | |||||||
Rank Position | geNorm | M-value | NormFinder | Variability | BestKeeper | Coeff. of corr. [r] | Consensus |
1 | PPIA | 0.21 | PPIA | 0.008 | PPIA | 0.99 | PPIA |
2 | GAPDH | 0.21 | GAPDH | 0.013 | GAPDH | 0.99 | GAPDH |
3 | Actin | 0.25 | Actin | 0.013 | Actin | 0.99 | Actin |
4 | RPII | 0.32 | RPII | 0.014 | RPII | 0.98 | RPII |
5 | TBP | 0.34 | TBP | 0.016 | TBP | 0.98 | TBP |
6 | 18s | 0.39 | 18s | 0.024 | 18s | 0.97 | 18s |