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Table 4 Gene ranking and consensus ranking using SYBR green super mixtures from two different vendors, Bio-Rad and Applied Biosystems

From: Reference gene validation for qPCR in rat carotid body during postnatal development

 

Gene and consensus ranking for group N1+N14 (n = 16) using Bio-Rad SYBR

 

Bio-Rad SYBR

Rank Position

geNorm

M-value

NormFinder

Variability

BestKeeper

Coeff. of corr. [r]

Consensus

1

TBP

0.26

GAPDH

0.01

PPIA

0.89

PPIA

2

RPII

0.26

PPIA

0.011

Actin

0.84

GAPDH

3

PPIA

0.27

Actin

0.014

GAPDH

0.83

Actin

4

Actin

0.34

TBP

0.015

18s

0.73

TBP

5

GAPDH

0.38

RPII

0.016

RPII

0.72

RPII

6

18s

0.40

18s

0.018

TBP

0.70

18s

 

Gene and consensus ranking for group N1 + N14 (n = 16) using Applied Biosystems SYBR

 

Applied Biosystems SYBR

Rank Position

geNorm

M-value

NormFinder

Variability

BestKeeper

Coeff. of corr. [r]

Consensus

1

PPIA

0.21

PPIA

0.008

PPIA

0.99

PPIA

2

GAPDH

0.21

GAPDH

0.013

GAPDH

0.99

GAPDH

3

Actin

0.25

Actin

0.013

Actin

0.99

Actin

4

RPII

0.32

RPII

0.014

RPII

0.98

RPII

5

TBP

0.34

TBP

0.016

TBP

0.98

TBP

6

18s

0.39

18s

0.024

18s

0.97

18s

  1. The reference genes are ranked for N1+N14 group by geNorm, Normfinder and BestKeeper and the consensus ranking determined by rank aggregation. (n) = number of independent determinations.