Table 1 Strains and plasmids used in this study
From: Escherichia coli BdcA controls biofilm dispersal in Pseudomonas aeruginosa and Rhizobium meliloti
Strain/Plasmid | Genotype | Source |
|---|---|---|
Strain | ||
E. coli BW25113 | lacI q rrnB T14 ΔlacZ WJ16 hsdR514 ΔaraBAD AH33 ΔrhaBAD LD78 | [34] |
E. coli DH5α | luxS supE44 ΔlacU169(Φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | |
E. coli DH5α/ pMMB206/pRK2013 | E. coli harboring pMMB206 and pRK2013 | This study |
E. coli DH5α/ pMMB206-BdcA/pRK2013 | E. coli harboring pMMB206-BdcA and pRK2013 | This study |
P. aeruginosa PA14 | P. aeruginosa wild-type strain | [36] |
P. fluorescens | P. fluorescens wild-type strain | R. Frazee |
R. meliloti 102F34 | R. meliloti wild-type strain | M. Sadowski |
PA14/pMMB206 | P. aeruginosa wild-type harboring pMMB206 | This study |
PA14/pMMB206-BdcA | P. aeruginosa wild-type harboring pMMB206-BdcA | This study |
PA14/ pMMB206/pP25-gfp | P. aeruginosa wild-type harboring pMMB206 and pP25-gfp | This study |
PA14/ pMMB206-BdcA/pP25-gfp | P. aeruginosa wild-type harboring pMMB206-BdcA and pP25-gfp | This study |
P. fluorescens/pMMB206 | P. fluorescens wild-type harboring pMMB206 | This study |
P. fluorescens/pMMB206-BdcA | P. fluorescens wild-type harboring pMMB206-BdcA | This study |
R. meliloti 102F34/pMMB206 | R. meliloti wild-type harboring pMMB206 | This study |
R. meliloti 102F34/pMMB206-BdcA | R. meliloti wild-type harboring pMMB206-BdcA | This study |
R. meliloti 102F34/pMMB206/pP25-gfp | R. meliloti wild-type harboring pMMB206 and pP25-gfp | This study |
R. meliloti 102F34/pMMB206-BdcA/pP25-gfp | R. meliloti wild-type harboring the pMMB206-BdcA and pP25-gfp | This study |
Plasmid | ||
pMMB206 | Cmr, Mob+, lacIq, broad-host range plasmid | [23] |
pMMB206-BdcA | Cmr; pMMB206 with P tac-lacUV5 ::bdcA | this work |
pRK2013 | Kmr; Tra+ Mob+ (RK2) Km::Tn7 ColEl origin, helper plasmid for mobilization | M. Bagdasarian, [37] |
pP25-gfp | Carr; constitutive green fluorescent protein production | [27] |