Invasion of Ureaplasma diversum in bovine spermatozoids

BMC Research Notes

Table 2 Determination of CCU/mL of U. diversum field isolates and ATCC strains for gentamicin invasion assay and relative frequencies of invasion.

Strains and isolates of U. diversum	Inoculums of tests (CCU/mL)^a	Inoculums after gentamicin exposure (CCU/mL)^a	Frequency of invasion (%)^b
ATCC 49782	55.00 ± 0.49	0.55 ± 0.4929	1.03 ± 0.92
ATCC 49783	550.00 ± 492.95	1.00	0.18
A203	5.50 ± 0.15	0.10	1.81
ALVA	100.00	1.00	1.00
94	550.00 ± 492.95	1.00	0.18
3175	1000.00	5.50 ± 4.92	0.55 ± 0.49
TOURO 1	1.00	0.10	10

^a Values CCU/mLx10⁴ of 3 independents tests performed in triplicate and standard deviations.
^b Percentage of ureaplasmas presenting change of colors in broth (CCU/mL) after gentamicin exposure compared to initial inoculums CCU/mL of test and standard deviations.

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