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Table 1 Number of hygromycin-resistant colonies expressing GFP at designated electroporation conditions.

From: Transformation of Epichloë typhina by electroporation of conidia

  

# GFP/total # HygR colonies

Overall

Ω

kV

1

2

3

4

GFP/Hyg

%GFP

400

1

26/28

23/35

18/24

20/26

87/113

77

400

1.25

36/45

28/37

26/39

41/48

131/169

78

400

1.5

15/19

18/21

17/20

27/32

77/92

84

600

1

25/33

30/37

19/27

21/26

95/123

77

600

1.25

20/25

14/19

20/23

15/19

69/86

80

600

1.5

6/10

17/20

10/14

5/10

38/54

70

800

1

27/41

18/27

18/22

25/33

88/123

72

800

1.25

12/15

4/5

7/8

9/10

32/38

84

800

1.5

9/10

12/14

10/13

9/10

40/47

85

  1. Electroporation was performed at combinations of three different resistances (400, 600, 800 Ω) and three different voltages (1.0, 1.25, and 1.5 kV) at 25 μF capacitance. The number of GFP expressing colonies and the total number of hygromycin-resistant colonies obtained from four different experiments at designated electroporation conditions are indicated in the table. Combined results of the four experiments are also presented.
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BMC Research Notes

ISSN: 1756-0500

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