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Table 1 Cytotoxic effect induced by transfection reagents

From: Comparative nucleic acid transfection efficacy in primary hepatocytes for gene silencing and functional studies

Reagents

Media

Cytotoxicity

  

24 hr

48 hr

72 hr

Negative control

DMEM

-

-

-

 

Opti-MEM

-

-

-

Metafectene Pro

DMEM

-

+

+

 

Opti-MEM

-

+

+

Lipofectamine 2000

DMEM

-

++

+++

 

Opti-MEM

-

+

++

Targefect

DMEM

+

+++

++++

 

Opti-MEM

-

++

++++

  1. Cytotoxicity was assessed by estimating the percentage of cell density in 6 fields per well, relative to the negative control for each time point. +: < 20%; ++: < 40%; +++: < 60%; ++++: < 80% cell death.
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BMC Research Notes

ISSN: 1756-0500

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