Figure 3From: An RNA isolation system for plant tissues rich in secondary metabolitesDenaturing gel electrophoresis of RNA isolated from different plant tissues using IHBT protocol. Name of plant tissues along with RNA yield (μg/100 mg tissue) is written above each panel; A260/280 ratio is mentioned at the bottom of the panel. The quality was assessed by electrophoresing an equal volume of RNA (2 μl) on formaldehyde-agarose denaturing gel to observe integrity of 28 S and 18 S rRNA bands, and by confirming the amenability of isolated RNA to reverse transcription-polymerase chain reaction based amplification of 26 S rRNA. Tea (Camellia sinensis, Family: Theaceae), potato (Solanum tuberosum, Family: Solanaceae), chili pepper (Capsicum annum, Family: Solanaceae), picrorhiza (Picrorhiza kurrooa, Family: Scrophulariaceae), stevia (Stevia rebaudiana, Family: Asteraceae), aromatic turmeric (Curcuma aromatica, Family: Zingiberaceae), and lantana (Lantana camara, Family: Verbenaceae) is rich in flavanoids, polysaccharides, capsaicinoids, picrosides, steviosides, curcuminoids, and triterpenoids, respectively. Arabidopsis (Arabidopsis thaliana, Family: Brassicaceae) is a model plant, whereas the knowledge on the nature of secondary metabolites in caragana (Caragana jubata, Family: Fabaceae), Himalayan cinquefoil (Potentilla atrosanguinea, Family: Rosaceae), cotton (Gossypium hirsutum, Family: Malvaceae), toona (Toona sinensis, Family: Meliaceae), and white clover (Trifolium repens, Family: Fabaceae) was not available.Back to article page