Table 1 Transcripts abundantly expressed in the striped bass ovary.
| Â | Contig Number | BLAST 2GO Annotation | Gene | GeneID zebrafish taxid: 7955 orthologue | Assembled contig length (bp) | Number of observe sequence reads | % Total sequence reads (230,151) | Fraction of ESTs that mapped to the zebrafish UniGene by DDD | Zebrafish UniGene | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | Â | Â | Â | Â | Â | Ovary | Â | Body | Â |
1 | 10186 | cyclin b2 | ccnb2 | 368316 | 1284 | 1146 | 0.4979340 | 0.0025 | > | 0.0001 | Dr.80580 |
2 | 10415 | zona pellucida glycoprotein | zp2.3 | 114439 | 1329 | 1076 | 0.4675192 | 0.0429 | > | 0.0012 | Dr.143785 |
3 | 10181 | novel protein with zona pellucida-like domain | si: ch211-14a17.7 | 368669 | 646 | 1001 | 0.4349318 | 0.0015 | > | 0.0001 | Dr.75717 |
4 | 9349 | zona pellucida c | zpcx | 334011 | 2036 | 923 | 0.4010411 | 0.0013 | > | 0.0001 | Dr.80433 |
5 | 146 | nad h quinone 1 | nqo1 | 322506 | 916 | 908 | 0.3945236 | n.d. | = | n.d. | Dr.4189 |
6 | 8878 | tubulin beta 2c | zgc: 123194 | 641421 | 1510 | 869 | 0.3775782 | n.d. | = | n.d. | Dr.52550 |
7 | 9768 | egg envelope component zpax | si: dkeyp-50f7.2 | 334036 | 2890 | 864 | 0.3754057 | 0.0017 | > | 0.0003 | Dr.105787 |
8 | 10472 | fatty acid binding protein liver | fabp1b.1 | 554095 | 419 | 848 | 0.3684538 | n.d. | = | n.d. | Dr.24261 |
9 | 9294 | --NA-- | -- | -- | 812 | 839 | 0.3645433 | -- | Â | -- | -- |
10 | 10137 | choriogenin 1 | zp3b | 64692 | 1389 | 817 | 0.3549843 | 0.0029 | > | 0.0003 | Dr.75734 |
11 | 11102 | hypothetical protein LOC100049339 | polr2a | 553347 | 774 | 767 | 0.3332595 | * | Â | * | Dr.79109 |
12 | 11074 | --NA-- | -- | -- | 181 | 762 | 0.3310870 | -- | Â | -- | -- |
13 | 10663 | zgc: 175135 protein | zgc: 165551 | 100003969 | 636 | 706 | 0.3067551 | 0.0039 | > | 0.0003 | Dr.106137 |
14 | 9917 | heat shock protein 8 | hspa8 | 573376 | 2266 | 699 | 0.3037136 | 0.0011 | < | 0.0029 | Dr.75087 |
15 | 11091 | novel protein with zona pellucida-like domain | LOC100331707 | 100331707 | 1219 | 675 | 0.2932857 | -- | Â | -- | -- |
16 | 3 | --NA-- | -- | -- | 1585 | 654 | 0.2841613 | -- | Â | -- | -- |
17 | 11147 | fatty acid-binding heart | fabp11a | 447944 | 581 | 638 | 0.2772093 | n.d. | = | n.d. | Dr.78045 |
18 | 10883 | mgc86501 protein | wu: ft38e01 | 798996 | 568 | 623 | 0.27069919 | 0.0024 | > | 0.0002 | Dr.106837 |
19 | 9329 | histone | h3f3c | 336231 | 945 | 619 | 0.2689539 | 0.0001 | < | 0.0003 | Dr.75577 |
20 | 10302 | voltage gated chloride channel domain-containing protein | -- | -- | 996 | 616 | 0.2676504 | -- | Â | -- | -- |
21 | 11112 | egg envelope component zpc | zp3c | 563179 | 1527 | 610 | 0.2650434 | 0.0002 | > | 0 | Dr.113688 |
22 | 30 | histone h2a | LOC573838 (h2af1o) | 100332229 | 447 | 607 | 0.2637399 | 0.0024 | > | 0.0002 | Dr.75698 |
23 | 10079 | --NA-- | -- | -- | 811 | 585 | 0.2541810 | -- | Â | -- | -- |
24 | 10058 | beta-actin | bactin2 | 57935 | 1874 | 578 | 0.2511395 | 0.0026 | < | 0.0077 | Dr.75125 |
25 | 10823 | apolipoprotein d | zgc: 123339 | 567972 | 816 | 560 | 0.2433185 | * | Â | * | Dr.15815 |
26 | 10825 | --NA-- | -- | -- | 154 | 555 | 0.2411460 | -- | Â | -- | -- |
27 | 10773 | hypothetical protein LOC100049339 | -- | 30705 | 756 | 555 | 0.2411460 | -- | Â | -- | -- |
28 | 6635 | h1 histone member oocyte-specific | h1m | 327403 | 823 | 523 | 0.2272421 | n.d. | = | n.d. | Dr.75735 |
29 | 11098 | adp atp translocase | slc25a5 | 192321 | 1243 | 515 | 0.2237661 | 0.0015 | < | 0.0078 | Dr.30295 |
30 | 127 | nucleoside diphosphate kinase b | nme2b.1 | 30083 | 834 | 511 | 0.2220281 | n.d. | = | n.d. | Dr.11052 |
| Â | Contig Number | BLAST 2GO Annotation | Gene | GeneID zebrafish taxid: 7955 Orthologue | Assembled contig length (bp) | Number of observe sequence reads | % Total sequence reads (230,151) | Fraction of ESTs that mapped to the zebrafish UniGene by DDD | Zebrafish UniGene | ||
| Â | Â | Â | Â | Â | Â | Â | Â | Ovary | Â | Body | Â |
31 | 10309 | 60 s acidic ribosomal protein p0 | rplp0 | 58101 | 932 | 497 | 0.2159452 | 0.0008 | < | 0.0033 | Dr.55617 |
32 | 11081 | loc494706 protein (oogenesis-related gene) | org | 100001110 | 601 | 495 | 0.2150762 | 0.0016 | > | 0.0001 | Dr.80745 |
33 | 10120 | elongation factor 1 alpha | efla | 30516 | 1744 | 492 | 0.2137727 | 0.0032 | < | 0.0108 | Dr.31797 |
34 | 10015 | heat shock protein 90 | hsp90ab1 | 30573 | 1900 | 485 | 0.2107312 | 0.0006 | < | 0.0020 | Dr.35688 |
35 | 11073 | unnamed protein product | -- | -- | 414 | 481 | 0.2089932 | -- | Â | -- | -- |
36 | 10797 | complement component (3b 4b) receptor 1 | LOC565541 | 565541 | 1696 | 470 | 0.2042138 | * | Â | * | Dr.91858 |
37 | 92 | cyclin b1 | ccnb1 | 58025 | 738 | 470 | 0.2042138 | 0.0035 | > | 0.0002 | Dr.121261 |
38 | 10403 | --NA-- | -- | -- | 327 | 469 | 0.2037793 | -- | Â | -- | -- |
39 | 126 | karyopherin alpha 2 (rag cohort importin alpha 1) | zgc: 55877 | 406343 | 1085 | 469 | 0.2037793 | 0.0010 | > | 0.0002 | Dr.20877 |
40 | 10948 | --NA-- | -- | -- | 248 | 465 | 0.2020413 | -- | Â | -- | -- |
41 | 10900 | zpb protein | LOC100334275 | 100334275 | 1561 | 461 | 0.2003033 | * | Â | * | Dr.141250 |
42 | 36 | claudin 4 | cldnd | 81583 | 731 | 456 | 0.1981308 | 0.0004 | > | 0.0001 | Dr.75663 |
43 | 216 | stathmin 1 oncoprotein 18 variant 8 | stmn1b | 550548 | 964 | 450 | 0.1955238 | 0 | < | 0.0004 | Dr.105609 |
44 | 10949 | --NA-- | -- | 550134 | 151 | 420 | 0.1824889 | -- | Â | -- | -- |
45 | 9337 | Securin [Anoplopoma fimbria] | LOC566690 | 566690 | 435 | 414 | 0.1798819 | 0.0002 | > | 0 | Dr.118007 |
46 | 9321 | dna replication inhibitor | gmnn | 368320 | 1121 | 412 | 0.1790129 | n.d. | = | n.d. | Dr.119358 |
47 | 10986 | cell division cycle 20 homolog (cerevisiae) | cdc20 | 406353 | 1597 | 410 | 0.1781439 | 0.0005 | > | 0.0001 | Dr.105018 |
48 | 11071 | --NA-- | -- | -- | 215 | 402 | 0.1746679 | -- | Â | -- | -- |
49 | 10743 | --NA-- | -- | -- | 273 | 398 | 0.1729299 | -- | Â | -- | -- |
50 | 1174 | cyclin k | LOC100331304 | 100331304 | 3331 | 397 | 0.1724954 | 0.0009 | > | 0 | Dr.148591 |
51 | 10438 | ribonucleotide reductase m2 polypeptide | rrm2 | 30733 | 1621 | 396 | 0.1720610 | 0.0018 | > | 0.0003 | Dr.75098 |
52 | 11198 | ribosomal protein s20 | rps20 | 406485 | 477 | 393 | 0.1707575 | 0.0014 | > | 0.0008 | Dr.18943 |
53 | 11014 | karyopherin alpha 2 (rag cohort importin alpha 1) | kpna2 | 436607 | 534 | 380 | 0.1651090 | 0.0009 | > | 0.0002 | Dr.75709 |
54 | 10351 | --NA-- | -- | -- | 299 | 375 | 0.1629365 | -- | Â | -- | -- |
55 | 10265 | unnamed protein product | -- | -- | 1075 | 375 | 0.1629365 | -- | Â | -- | -- |
56 | 771 | cytochrome c oxidase copper chaperone | cox17 | 447914 | 410 | 375 | 0.1629365 | 0.0007 | > | 0.0001 | Dr.82168 |
57 | 10107 | tubulin, alpha 1c | MGC171407 | 573122 | 697 | 374 | 0.1625020 | n.d. | = | n.d. | Dr.120425 |
58 | 161 | --NA-- | -- | -- | 2532 | 371 | 0.1611985 | -- | Â | -- | -- |
59 | 231 | epididymal secretory protein e1 precursor | npc2 | 282673 | 728 | 360 | 0.1564190 | -- | Â | -- | -- |
60 | 11090 | --NA-- | -- | -- | 308 | 356 | 0.1546811 | -- | Â | -- | -- |
| Â | Contig Number | BLAST 2GO Annotation | Gene | GeneID zebrafish taxid: 7955 orthologue | Assembled contig length (bp) | Number of observed sequence reads | % Total sequence reads (230,151) | Fraction of ESTs that mapped to the zebrafish UniGene by DDD | Zebrafish UniGene | ||
| Â | Â | Â | Â | Â | Â | Â | Â | Ovary | Â | Body | Â |
61 | 10741 | ppia protein (pepitidylprolyl isomerase A) | ppia | 336612 | 825 | 356 | 0.1546811 | 0.0005 | < | 0.0011 | Dr.104642 |
62 | 9354 | superoxide dismutase | sod1 | 30553 | 795 | 356 | 0.1546811 | n.d. | = | n.d. | Dr.75822 |
63 | 10048 | ubiquitin b | ubb | 550134 | 169 | 355 | 0.1542466 | n.d. | = | n.d. | Dr. 104259 |
64 | 10083 | cyclin a2 | ccna2 | 192295 | 2108 | 351 | 0.1525086 | n.d. | = | n.d. | Dr.121874 |
65 | 10746 | eukaryotic translation elongation factor 1 gamma | eef1g | 195822 | 1533 | 350 | 0.1520741 | 0.0006 | < | 0.0011 | Dr.75657 |
66 | 10761 | egg envelope component zpax | si: dkeyp-50f7.2 | 334036 | 2731 | 347 | 0.1507706 | 0.0017 | > | 0.0003 | Dr.105787 |
| Â | Â | Â | Â | TOTALS | 69173 | 36532 | 15.8730570 | Â | Â | Â | Â |