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Table 1 Transcripts abundantly expressed in the striped bass ovary.

From: An ovary transcriptome for all maturational stages of the striped bass (Morone saxatilis), a highly advanced perciform fish

  Contig Number BLAST 2GO Annotation Gene GeneID zebrafish taxid: 7955 orthologue Assembled contig length (bp) Number of observe sequence reads % Total sequence reads (230,151) Fraction of ESTs that mapped to the zebrafish UniGene by DDD Zebrafish UniGene
         Ovary   Body  
1 10186 cyclin b2 ccnb2 368316 1284 1146 0.4979340 0.0025 > 0.0001 Dr.80580
2 10415 zona pellucida glycoprotein zp2.3 114439 1329 1076 0.4675192 0.0429 > 0.0012 Dr.143785
3 10181 novel protein with zona pellucida-like domain si: ch211-14a17.7 368669 646 1001 0.4349318 0.0015 > 0.0001 Dr.75717
4 9349 zona pellucida c zpcx 334011 2036 923 0.4010411 0.0013 > 0.0001 Dr.80433
5 146 nad h quinone 1 nqo1 322506 916 908 0.3945236 n.d. = n.d. Dr.4189
6 8878 tubulin beta 2c zgc: 123194 641421 1510 869 0.3775782 n.d. = n.d. Dr.52550
7 9768 egg envelope component zpax si: dkeyp-50f7.2 334036 2890 864 0.3754057 0.0017 > 0.0003 Dr.105787
8 10472 fatty acid binding protein liver fabp1b.1 554095 419 848 0.3684538 n.d. = n.d. Dr.24261
9 9294 --NA-- -- -- 812 839 0.3645433 --   -- --
10 10137 choriogenin 1 zp3b 64692 1389 817 0.3549843 0.0029 > 0.0003 Dr.75734
11 11102 hypothetical protein LOC100049339 polr2a 553347 774 767 0.3332595 *   * Dr.79109
12 11074 --NA-- -- -- 181 762 0.3310870 --   -- --
13 10663 zgc: 175135 protein zgc: 165551 100003969 636 706 0.3067551 0.0039 > 0.0003 Dr.106137
14 9917 heat shock protein 8 hspa8 573376 2266 699 0.3037136 0.0011 < 0.0029 Dr.75087
15 11091 novel protein with zona pellucida-like domain LOC100331707 100331707 1219 675 0.2932857 --   -- --
16 3 --NA-- -- -- 1585 654 0.2841613 --   -- --
17 11147 fatty acid-binding heart fabp11a 447944 581 638 0.2772093 n.d. = n.d. Dr.78045
18 10883 mgc86501 protein wu: ft38e01 798996 568 623 0.27069919 0.0024 > 0.0002 Dr.106837
19 9329 histone h3f3c 336231 945 619 0.2689539 0.0001 < 0.0003 Dr.75577
20 10302 voltage gated chloride channel domain-containing protein -- -- 996 616 0.2676504 --   -- --
21 11112 egg envelope component zpc zp3c 563179 1527 610 0.2650434 0.0002 > 0 Dr.113688
22 30 histone h2a LOC573838 (h2af1o) 100332229 447 607 0.2637399 0.0024 > 0.0002 Dr.75698
23 10079 --NA-- -- -- 811 585 0.2541810 --   -- --
24 10058 beta-actin bactin2 57935 1874 578 0.2511395 0.0026 < 0.0077 Dr.75125
25 10823 apolipoprotein d zgc: 123339 567972 816 560 0.2433185 *   * Dr.15815
26 10825 --NA-- -- -- 154 555 0.2411460 --   -- --
27 10773 hypothetical protein LOC100049339 -- 30705 756 555 0.2411460 --   -- --
28 6635 h1 histone member oocyte-specific h1m 327403 823 523 0.2272421 n.d. = n.d. Dr.75735
29 11098 adp atp translocase slc25a5 192321 1243 515 0.2237661 0.0015 < 0.0078 Dr.30295
30 127 nucleoside diphosphate kinase b nme2b.1 30083 834 511 0.2220281 n.d. = n.d. Dr.11052
  Contig Number BLAST 2GO Annotation Gene GeneID zebrafish taxid: 7955 Orthologue Assembled contig length (bp) Number of observe sequence reads % Total sequence reads (230,151) Fraction of ESTs that mapped to the zebrafish UniGene by DDD Zebrafish UniGene
         Ovary   Body  
31 10309 60 s acidic ribosomal protein p0 rplp0 58101 932 497 0.2159452 0.0008 < 0.0033 Dr.55617
32 11081 loc494706 protein (oogenesis-related gene) org 100001110 601 495 0.2150762 0.0016 > 0.0001 Dr.80745
33 10120 elongation factor 1 alpha efla 30516 1744 492 0.2137727 0.0032 < 0.0108 Dr.31797
34 10015 heat shock protein 90 hsp90ab1 30573 1900 485 0.2107312 0.0006 < 0.0020 Dr.35688
35 11073 unnamed protein product -- -- 414 481 0.2089932 --   -- --
36 10797 complement component (3b 4b) receptor 1 LOC565541 565541 1696 470 0.2042138 *   * Dr.91858
37 92 cyclin b1 ccnb1 58025 738 470 0.2042138 0.0035 > 0.0002 Dr.121261
38 10403 --NA-- -- -- 327 469 0.2037793 --   -- --
39 126 karyopherin alpha 2 (rag cohort importin alpha 1) zgc: 55877 406343 1085 469 0.2037793 0.0010 > 0.0002 Dr.20877
40 10948 --NA-- -- -- 248 465 0.2020413 --   -- --
41 10900 zpb protein LOC100334275 100334275 1561 461 0.2003033 *   * Dr.141250
42 36 claudin 4 cldnd 81583 731 456 0.1981308 0.0004 > 0.0001 Dr.75663
43 216 stathmin 1 oncoprotein 18 variant 8 stmn1b 550548 964 450 0.1955238 0 < 0.0004 Dr.105609
44 10949 --NA-- -- 550134 151 420 0.1824889 --   -- --
45 9337 Securin [Anoplopoma fimbria] LOC566690 566690 435 414 0.1798819 0.0002 > 0 Dr.118007
46 9321 dna replication inhibitor gmnn 368320 1121 412 0.1790129 n.d. = n.d. Dr.119358
47 10986 cell division cycle 20 homolog (cerevisiae) cdc20 406353 1597 410 0.1781439 0.0005 > 0.0001 Dr.105018
48 11071 --NA-- -- -- 215 402 0.1746679 --   -- --
49 10743 --NA-- -- -- 273 398 0.1729299 --   -- --
50 1174 cyclin k LOC100331304 100331304 3331 397 0.1724954 0.0009 > 0 Dr.148591
51 10438 ribonucleotide reductase m2 polypeptide rrm2 30733 1621 396 0.1720610 0.0018 > 0.0003 Dr.75098
52 11198 ribosomal protein s20 rps20 406485 477 393 0.1707575 0.0014 > 0.0008 Dr.18943
53 11014 karyopherin alpha 2 (rag cohort importin alpha 1) kpna2 436607 534 380 0.1651090 0.0009 > 0.0002 Dr.75709
54 10351 --NA-- -- -- 299 375 0.1629365 --   -- --
55 10265 unnamed protein product -- -- 1075 375 0.1629365 --   -- --
56 771 cytochrome c oxidase copper chaperone cox17 447914 410 375 0.1629365 0.0007 > 0.0001 Dr.82168
57 10107 tubulin, alpha 1c MGC171407 573122 697 374 0.1625020 n.d. = n.d. Dr.120425
58 161 --NA-- -- -- 2532 371 0.1611985 --   -- --
59 231 epididymal secretory protein e1 precursor npc2 282673 728 360 0.1564190 --   -- --
60 11090 --NA-- -- -- 308 356 0.1546811 --   -- --
  Contig Number BLAST 2GO Annotation Gene GeneID zebrafish taxid: 7955 orthologue Assembled contig length (bp) Number of observed sequence reads % Total sequence reads (230,151) Fraction of ESTs that mapped to the zebrafish UniGene by DDD Zebrafish UniGene
         Ovary   Body  
61 10741 ppia protein (pepitidylprolyl isomerase A) ppia 336612 825 356 0.1546811 0.0005 < 0.0011 Dr.104642
62 9354 superoxide dismutase sod1 30553 795 356 0.1546811 n.d. = n.d. Dr.75822
63 10048 ubiquitin b ubb 550134 169 355 0.1542466 n.d. = n.d. Dr. 104259
64 10083 cyclin a2 ccna2 192295 2108 351 0.1525086 n.d. = n.d. Dr.121874
65 10746 eukaryotic translation elongation factor 1 gamma eef1g 195822 1533 350 0.1520741 0.0006 < 0.0011 Dr.75657
66 10761 egg envelope component zpax si: dkeyp-50f7.2 334036 2731 347 0.1507706 0.0017 > 0.0003 Dr.105787
     TOTALS 69173 36532 15.8730570     
  1. Genes are ranked (1-66) by number of observed 454 short read sequences used in each contig assembly. Digital Differential Display (DDD) results of orthologous sequences in zebrafish are also shown
  2. Annotation "--NA--"indicates no blastx return; Dashes (--) indicate unknown or data not available; asterisks (*) indicate the UniGene was not present in the EST libraries used for DDD. Sequences with expression differences evaluated by DDD (FET, P ≤ 0.05) are indicated by ">" (enhanced ovary expression) or "<" (enhanced body expression); "n.d." indicates no significant difference in expression between ovary and body (=)