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Table 1 Nuclear protein extract concentration and quality estimated by the contamination by non nuclear (cytosolic) fraction nd: not detected; the same letter indicates that values are not significantly different (P > 0.05). Value is mean ± standard error

From: Isolation of nuclear proteins from flax (Linum usitatissimum L.) seed coats for gene expression regulation studies

 

Commercial kit

Published method 1[10]

Published method 2[11]

Optimized protocol (present study)

Protein concentration (μg.mg -1 FW)

168.5 ± 7.2a

188.2 ± 6.6b

173.8 ± 9.0b

247.8 ± 6.1 c

AdH specific activity (AU.mg -1 FW)

20.7 ± 3.0c

8.8 ± 1.2bc

15.9 ± 3.8c

nd a