Table 1 Primers used in genome walking PCR
From: Cloning and expression of an endo-1,4-β-xylanase from the coffee berry borer, Hypothenemus hampei
Primers | Sequence (5'-3') |
|---|---|
GSP1_XIL_GW1_3 | CCAATTCATGGAGTCGGTTTCCAATCTCAT |
GSP1_XIL_GW1_5 | TTCGTTGAAGATTTCGTTGACTACATCCCA |
GSP2_XIL_GW1_3 | GTTGATGTGGCCATTACTGAATTGGACATC |
GSP2_XIL_GW1_5 | GGCGTCCCATTTCATTTCATTTTCTGGTGT |
GSP1_XIL_GW2_3 | GATTTAGCAATTCCCGAATGATGTTCACAC |
GSP1_XIL_GW2_5 | CCAAGTACTTTGGCCCAGTAAACACGAAAA |
GSP2_XIL_GW2_3 | CAAACAGTTACACGCCGGGGTAACTCCCAT |
GSP2_XIL_GW2_5 | CACGGAAGCGTTTTCGAGATATGAATG |
GSP1_XIL_GW3_3 | CGGAATGCGATAGAAAGGGATGGTTAAC |
GSP1_XIL_GW3_5 | TCAAGTGTTCCCTATCAGGAAATTGAC |
GSP2_XIL_GW3_3 | GTCGTCATTTATAGTCTTCCCTGAAGTTG |
GSP2_XIL_GW3_5 | CTAGTTCGTCAACTGCACAATGCCTGT |