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Figure 5 | BMC Research Notes

Figure 5

From: Ribozyme-mediated gene knock down strategy to dissect the consequences of PDGF stimulation in vascular smooth muscle cells

Figure 5

Phosphorylated protein comparative analyses. A) Histogram of PDI-A3 phosphorylation . B) Histogram of HSP-60 phosphorylation. Untreated: refers to quiescent VSMCs. Gray scale value of untreated samples was conventionally assumed to be 1 as normalization factor; PDGF: refers to VSMCs stimulated with PDGF-BB; PDGF+active ribozyme and PDGF+inactive ribozyme: refer to VSMCs cultured as before and additionally treated with active or inactive ribozyme. Bars represent the mean ± SE of at least three independent experiments. Data were statistically elaborated using the unpaired t- test (* P < 0.05, ** P < 0.01, *** P < 0.001)

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