Figure 1

Reduction of interspot contamination in HeLa cell arrays. (a) Picture of a spotted LabTek chamber. Schematic of locations of plasmids encoding for mCitrine and mCherry (green and red spots), and unspotted (empty) areas are shown in the lower panel. Contaminants expressing the opposite fluorescent protein in mCitrine/mCherry spots or in unspotted areas are shown in the right schematic. (b) and (c) Montage of fields (spots or empty areas) of half arrays containing mCitrine/mCherry-expressing HeLa cells. 20 min after cell seeding a wash step was included in (c) but not in (b). Reverse transfection time is indicated. Right panels show magnifications of representative spots.