Figure 3

Cell dynamics in spots. MCF7 (a) or HeLa (b) cells seeded according to the standard protocol without washing step and incubated for 39 hours. After the first 24 hours of incubation cells were tracked in time lapse live cell imaging for 15 hours under growing conditions. The merged image (mCitrine in green and mCherry in red) of representative frames acquired every 2:30 hours are shown. (c) A431D cells were incubated for 5 hours after cell seeding and monitored for 7 hours. The merged image of the transmission, mCitrine (green) and mCherry (red) channels, taken every 75 min are shown.