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Table 1 List and description of candidate reference genes for qPCR

From: Evaluation of qPCR reference genes in two genotypes of Populus for use in photoperiod and low-temperature studies

Symbol

Locus name Phytozome v2.2

At ortholog accession no.

Gene name

Function

Reference

TIP4-like

POPTR_0009s09620

NM_119592

TIP4-like

Putative cytoskeletal protein

[15]

CYC063

POPTR_0005s26170

AY652862a

Cyclophilin

Peptidylprolyl isomerase, protein folding

[25]

PT1

POPTR_0014s03160

NM_119492

Unknown protein

Unknown function, expressed in pollen tube cells

[15]

CDC2

POPTR_0004s14080

NM_114734

Cell division control protein 2

Cyclin-dependent kinase 2

[26]b

ACT2

POPTR_0001s31700

AB067722

Actin 2

Formation of filaments, component of cytoskeleton

[26]b

18S rRNA

Scaffold 17

AY652861a

18S ribosomal RNA

Constituent of ribosome

[25, 27]

ANT

POPTR_0014s01260

AY117207

AINTEGUMENTA

Putative ovule development protein

[15]b

UBQ7

POPTR_0005s09940

NM_129118

Ubiquitin

Protein modification, ubuquitin-dependant protein catabolism

[22]

  1. Gene symbol, Populus locus name (Phytozome), NCBI Arabidopsis thaliana ortholog accession number, gene name, function (annotation from Phytozome) and reference for each gene.
  2. a accession number for Populus.
  3. b primers were not redesigned.