Table 2 Characteristics of qPCR primers pairs for candidate reference genes
Gene | Primers (5'-3') | Product size (bp) | Annealing temp (°C) | P. trichocarpa PCR efficiency | P. tremula x P. alba PCR efficiency |
|---|---|---|---|---|---|
TIP4-like | F: GCTGATAATGGGGTGTCG R: CAACTCTAAGCCAGAATCGC | 88 | 57 | 1.969 | 2.018 |
CYC063 | F: CCTGGCACTAATGGGTCTCAG R: CACAACTCTTCCGAACACCAC | 87 | 52 | 1.98 | 1.978 |
PT1 | F: GCGGAAAGAAAAACTGCAAG R: TGACAGCACAGCCCAATAAG | 126 | 57 | 2.025 | 2.083 |
CDC2 | F: ATTCCCCAAGTGGCCTTCTAAG R: TATTCATGCTCCAAAGCACTCC | 137 | 57 | 2.04 | 2.035 |
ACT2 | F: TTCTACAAGTGCTTTGATGGTGAGTTC R:CTATTCGATACATAGAAGATCAGAATGTTC | 159 | 52 | 1.935 | 1.951 |
18S rRNA | F: GATTCTATGGGTGGTGGTGC R: CAGGCTGAGGTCTCGTTCG | 87 | 60 | 1.951 | 1.965 |
ANT | F: TCTGTCTGTTATGCCCCTCA R: CCACCTAGGAAGTCCTCCAGT | 119 | 55 | 2.062 | 2.033 |
UBQ7 | F: GGAACGGGTTGAGGAGAAAGAAG R: GCAAGAACAAGATGAAGCACAGAGC | 135 | 55 | 2.028 | 2.016 |