Table 2 Primer sequences and nucleotide dispensation order used in pyrosequencing assays
From: Copy number variations of the ATP-binding cassette transporter ABCC6 gene and its pseudogenes
Assay # | Genes | Template | Primer | Sequence | Dispensation order | Amplicon size (bp) |
|---|---|---|---|---|---|---|
123456789 | ||||||
1 | ABCC6-ABCC6P1 | gDNA | Forward | 5’-TGAGGGAGCCAGGCTAGA-3’ | 129 | |
Reverse | 5’-Biotin-GAGGGGAAGGGAGAGATTAGC-3’ | |||||
Sequencing | 5’-GCCTGGCCCTGCCGC-3’ | GTAGC TGC T | ||||
2 | ABCC6-ABCC6P1-ABCC6P2 | gDNA | Forward | 5’-Biotin-TCCCATCTACCTCCTCTTCATC-3’ | 76 | |
Reverse | 5’-ATCTTGGCTTTGAAGAGTGG-3’ | |||||
Sequencing | 5’-TGGCTTTGAAGAGTGG-3’ | CGACAT CT | ||||
3 | ABCC6-ABCC6P1-ABCC6P2 | cDNA | Forward | 5’-Biotin-CGGGGCAGGGGGTCTGGAAC-3’ | 195 | |
Reverse | 5’-ATCTTGGCTTTGAAGAGTGG-3’ | |||||
Sequencing | 5’-TGGCTTTGAAGAGTGG-3’ | CGACAT CT |