Figure 3

RT-PCR and quantitative RT-PCR analysis. A: PCR analysis of cDNA transcribed from RNA extracted by the different columns. RT-PCR, Lanes 1 and 4, cDNA template was transcribed from RNA extracted using the common RNA extraction protocol [4] without column extraction step. Lanes 2 and 5, cDNA template was transcribed from RNA produced using the plasmid DNA extraction column. Lanes 3 and 6, cDNA template was transcribed from RNA produced using the RNA collection column (RNeasy Plant Mini Kit). EtOH, 96% ethanol added to samples before transfer through columns. Actin 1 (Act1, lanes 1-3) and β-Tubulin 2 (Tub2, lanes 4-6) primers were used. M, DNA 1-kb ladder. B: Quantitative real-time RT-PCR on cDNA transcribed from RNA produced using LogSpin protocol, amplification plot (upper panel) and fold change of AtPR1 gene normalized to AtPTB1F on samples from inoculated versus uninoculated (control) Arabidopsis leaves. Relative AtPR1 gene expression was calculated by the 2^-ΔΔCt method (ΔRn).