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Table 2 Bacterial strains and plasmids used in this study

From: A single copy integration vector that integrates at an engineered site on the Staphylococcus aureus chromosome

Strain or Plasmid

Relevant Characteristics

Reference or Source

Staphylococcus aureus

  

RN4220

Restriction deficient laboratory strain

[26]

CYL12337

RN4220 attB 1

This study

CYL12348

RN4220 attB 2

This study

CYL12349

CYL12348 (pYL112Δ19)

This study

CYL12376

CYL12349 (pLL102)

This study

E. coli

  

DH5α

Strain for cloning and plasmid maintenance

Invitrogen

XL1-Blue

Strain for cloning and plasmid maintenance

Stratagene

Plasmids

  

pCL52.2

Temperature-sensitive cloning vector

[27]

pLL3961

Derivative of pCL52.2 carrying attP 1 and the L54a int gene

This study

pCL25

Carries wild-type attP of L54a

[2]

pKOR1

Used for allele replacement in S. aureus

[21]

pYL112Δ19

Encodes integrase of phage L54a

[3]

pLL102

Derivative of pCL25 carrying attP 2

This study