Strain or Plasmid | Relevant Characteristics | Reference or Source |
---|---|---|
Staphylococcus aureus | Â | Â |
RN4220 | Restriction deficient laboratory strain | [26] |
CYL12337 | RN4220 attB 1 | This study |
CYL12348 | RN4220 attB 2 | This study |
CYL12349 | CYL12348 (pYL112Δ19) | This study |
CYL12376 | CYL12349 (pLL102) | This study |
E. coli | Â | Â |
DH5α | Strain for cloning and plasmid maintenance | Invitrogen |
XL1-Blue | Strain for cloning and plasmid maintenance | Stratagene |
Plasmids | Â | Â |
pCL52.2 | Temperature-sensitive cloning vector | [27] |
pLL3961 | Derivative of pCL52.2 carrying attP 1 and the L54a int gene | This study |
pCL25 | Carries wild-type attP of L54a | [2] |
pKOR1 | Used for allele replacement in S. aureus | [21] |
pYL112Δ19 | Encodes integrase of phage L54a | [3] |
pLL102 | Derivative of pCL25 carrying attP 2 | This study |