Ubiquinol decreases monocytic expression and DNA methylation of the pro-inflammatory chemokine ligand 2 gene in humans

Table 2 Position, length and number of CpG islands of amplicons covering the analysed genomic regions of the human CXCL2, PMAIP1 and MMD gene

	start	end	length	CpGs	left primer (+Tag)	right primer (+Tag)
*gene/amplicon*
PMAIP1_amp01 f	3	565	563	57	ATTGTTAAGGTTTTTGGTTTTTTTT	CTCAACCTCCAACTAAAACACCTC
PMAIP1_amp02 f	485	1068	584	49	TTTTAGTATTTTTGTTTGTAGGATTGTT	AAACTCTCTCCTACCCCTTCTACC
PMAIP1_amp03 f	1113	1529	417	13	AGGGTTTTTGTGTTTAGGAGTTTAGA	AAATAAACAAAACTTTTTCCATCCC
PMAIP1_amp07 f	-23	565	587	57	TTTGGGTTTGTTTATTTAAGTTTTT	CTCAACCTCCAACTAAAACACCTC
PMAIP1_amp04 r	3	512	510	53	AATAGTTTTGTAGGTAGGGATGTTGG	ATTACCAAAACCTCTAATCTCTCCC
PMAIP1_amp05 r	374	954	581	53	AGGAGGAAAGGAGTTTTTTGTTTTT	TCACCAAAAAAATTCTCACTAAACA
PMAIP1_amp06 r	930	1481	552	22	TTGTTATTAATTTAGGTATGGTTATATTTG	AAAAACAAAAAACTCCTTTCCTCCT
PMAIP1_amp08 r	-9	399	409	43	TTGTTTAGTGAGAATTTTTTTGGTG	CCCAAATCTCTAATTACCAAAACCT
MMD_amp01 f	236	986	751	81	AGGTAGGGTTGTTTGTTTGTTGTTA	AATCCACCCAAAATAAATCCAAAT
MMD_amp02 r	274	1015	742	82	TAGGGAATTGATTTTTGGTTAAGGT	ACTTTTAAAATTTCCTAATCCATCTCC
CXCL2_amp01 f	2	427	426	30	AGGATSGSTAAGATATGTTGTAGTTTTTG	CTTTTATACATAATTAAAACTAAAAAACCC
CXCL2_amp02 f	228	780	553	48	GGGGTAGAAAGAGAATATTTTATAGTTGG	AAATTCCCTACAAAATCTACAAACAC
CXCL2_amp03 r	45	491	447	34	GAGGAGAGTTGGTAAGGAGTTGTTT	CCCAACAACTAAAATATCTTCCAAAA
CXCL2_amp04 r	399	794	396	34	GATGTTTTTGAGGTGAATTTTTTGT	AACTTTCCAACCCCAACCATACATA

The start and end positions of amplicons refer to the genomic regions as illustrated in Figure 1 (CXCL2 gene) and Additional file 1: Figure S1 (PMAIP1) and Additional file 2: Figure S2 (MMD). Primer sequences shown in 5´ to 3´ direction are complementary to sequences obtained after bisulfite treatment and differ from the original genomic target by exchanging each “C” with “T”. f, forward direction of the amplicon; r, reverse direction of the amplicon.

ISSN: 1756-0500