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Table 4 Substrate specificity of Cel14b22

From: Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene

Substrates

Specific activitya(U/mg) ± SD

Relative activity (%)

Oat gum ((1–3,1-4)-β-D-glucan)

368.22 ± 2.77

100

Barley β-glucan ((1–3,1-4)-β-D-glucan)

172.94 ± 9.92

47

Carboxymethyl cellulose (1,4-β-D-glucan)

110.42 ± 4.07

30

Lichenan ((1–3,1-4)-β-D-glucan)

86.48 ± 4.45

23

Oat spelt xylan (1,4-β-D-xylan)

39.79 ± 3.62

11

Methyl cellulose (1,4-β-D-glucan)

39.41 ± 3.72

11

Birchwood xylan (1,4-β-D-xylan)

38.29 ± 5.44

10

Avicel (1,4-β-D-glucan)

21.96 ± 3.40

6

Filter paper

20.78 ± 6.97

6

  1. a The incubation time for activity measurement was 15 min, performed in triplicate and the standard deviations were calculated.