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Table 3 Comparison of the reliability of estimating A-to-I RNA editing of the Dα6 subunit using the peak height ratio method between Dα6IR2 and Dα6285F primers at different rates of expected editing

From: Antisense sequencing improves the accuracy and precision of A-to-I editing measurements using the peak height ratio method

 

Editing Site

Seq. Primer

398

400

415

416

Dα6IR2

0.98 ± 0.03

(1.00)

1.00 ± 0.03

(1.00)

0.97 ± 0.05

(1.00)

0.97 ± 0.05

(1.00)

Dα6285F

0.94 ± 0.10

(0.99)

0.88 ± 0.10*

(0.99)

0.80 ± 0.07*

(0.99)

0.85 ± 0.04*

(1.00)

  1. Values are the slopes and associated confidence intervals of editing estimate vs. expected values. Numbers in parentheses indicate the r2 value. Slopes with * indicate values that are significantly different than 1.0 (i.e., 95% CI does not include 1.0)