Figure 1From: Optimization of quantitative polymerase chain reactions for detection and quantification of eight periodontal bacterial pathogensAnalysis by mFold of the secondary structure of the Tannerella forsythia 16S rRNA gene amplicon, targeted by the primers described by Kuboniwa et al . [42]. Folding conditions were adapted to qPCR conditions (see 2.4). Forward primer anneals on bp 1–22 region, which contains a hairpin (bp 7–18).Back to article page