Salmo salar L. | n | Effects |
---|
Fixed | Random | DFn | DFd | Sum Sq | F | P |
---|
Weight [log] | 180 | Treatment | Treatment:Tank | 1 | 28 | 0.62 | 23.19 | <0.0001*** |
 |  | Type |  | 2 | 27 | 6.06 | 113.11 | <0.0001*** |
Gene | n | Â | Â | DFn | DFd | Â | F | P |
EF1A A
| 177 | Type | Treatment:Tank | 2 | 26 | 0.0002 | 1.17 | 0.3 |
HSP70
| 173 | Treatment | Treatment:Tank | 1 | 26 | 0.0017 | 0.72 | 0.3 |
GR
| 173 | Type | Treatment:Tank | 2 | 25 | 0.08 | 7.92 | 0.001*** |
Cu/Zn SOD | 166 | Type | Treatment:Tank | 2 | 24 | 0.06 | 3.49 | 0.04* |
Mn SOD
| 170 | Type | Weight [log] | 2 | 24 | 0.03 | 5.42 | 0.01** |
GP
| 172 | Type | Weight [log] | 2 | 24 | 0.07 | 6.79 | 0.003** |
IGF-1 | 168 | Treatment | Weight [log] | 1 | 25 | 0.09 | 13.57 | <0.001*** |
 |  | Type |  | 2 | 24 | 0.15 | 10.97 | <0.001*** |
IGF-1R | 173 | Type | Treatment:Tank | 2 | 23 | 0.01 | 1.37 | 0.24 |
- Summary of the best linear mixed effect models testing for difference in fish weight, and mRNA levels (raw Cq values of EF1AA, ΔΔCq values of target genes) between treatments and genetic origins (type). Including the interaction between treatment and type did not improve the fit of any of the models, thus all groups were affected by treatment in the same matter. The random effects were included by forward selection. Treatment:Tank; tank nested within treatment, Weight [log]; log transformed weight measurement of the fish. For more information on model selection based upon AIC values, see Additional file 1. The statistical significance is marked with asterisks, where * = P ≤ 0.05, ** = P ≤ 0.01 and *** = P ≤ 0.001.