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Figure 3 | BMC Research Notes

Figure 3

From: Transcriptome walking: a laboratory-oriented GUI-based approach to mRNA identification from deep-sequenced data

Figure 3

Paired ends effectively increase read length. A similar CaM-kinase walk display to Figure 2, but now the reads have been sorted to bring paired ends together, while retaining the match position to the growing sequence for one of each pair. Each read is still positioned horizontally according to its match position. The sorting algorithm also calculated the effective fragment length for each pair and gave estimates of the mean and range of fragment lengths.

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