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Figure 3 | BMC Research Notes

Figure 3

From: Phosphodiesterase inhibition mediates matrix metalloproteinase activity and the level of collagen degradation fragments in a liver fibrosis ex vivo rat model

Figure 3

A: C3M release from ex vivo cultures after 48 hr incubation. Liver slices from fibrotic or vehicle rats were cultured in Williams E medium and released C3M levels in the supernatants were measured in the C3M ELISA. W/o: Williams E medium without any intervention; IBMX: Williams E medium + 100 μM IBMX; GM6001: Williams E medium + 10 μM GM6001. Number of livers used (N): Vehicle N = 5 fibrotic N = 9. Number of liver slices used pr liver (n): n = 10 in each condition. Asterisks *p < 0.05, **p < 0.01. B: AlamarBlue was investigated as a measure of metabolic activity in fibrotic and vehicle liver slices with addition of IBMX or GM6001 in Williams E medium after 48 hour culturing period. W/o: Williams E medium without any intervention; IBMX: Williams E medium + 100 μM IBMX; GM6001: Williams E medium + 10 μM GM6001. Number of livers used (N): Vehicle N = 5, Fibrotic N = 9. Number of liver slices used pr liver (n): n = 10 in each condition. Asterisks **p < 0.01.

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