Figure 4

A: MMP expression and activity assessment by gelatin zymography. Liver explants were cultured without intervention (w/o) or with either 100 μM IBMX or 10 μM GM6001. The MMP activity in the supernatants after 48 hr is identified on the zymography gel by the standards for MMP-2 and MMP-9. Pro-MMP-9 and active MMP-9 are seen as bands migrating 92 kDa and 86 kDa, respectively, while pro-MMP-2 and active MMP-2 are seen as bands migrating 72 kDa and 66 kDa, respectively. B-C: Quantification of MMP-2 and MMP-9 activity in supernatants from healthy and fibrotic liver slices assessed by densitometry. Asterisks **p < 0.01.