Table 1 Real-time PCR systems used to test the efficiency of stool concentration by lyophilization
From: Optimized microbial DNA extraction from diarrheic stools
Bacteria | Target | Sequence (5’ – 3’) | Length (bp) |
|---|---|---|---|
Methanobrevibacter smithii | 16S rRNA | CCGGGTATCTAATCCGGTTC | 20 |
CTCCCAGGGTAGAGGTGAAA | 20 | ||
CCGTCAGAATCGTTCCAGTCAG | 22 | ||
All bacteria | 16S rRNA | AGAGTTTGATCMTGGCTCAG | 20 |
TTACCGCGGCKGCTGGCAC | 19 | ||
CCAKACTCCTACGGGAGGCAGCAG | 24 | ||
Salmonella enterica | Chorismate synthase | CAAGAAATACCTGGCGGAAA | 20 |
CGGGACAAAAGAACGGATTA | 20 | ||
GTTCGGCATCGAAATCCGCG | 20 |